Zebrafish Genetics and Formation of Embryonic Vasculature

The embryonic vasculature develops in a conserved manner in all vertebrates. Endothelial progenitor cells differentiate from mesodermal cells, then migrate and assemble into the dorsal aorta and the cardinal vein. This primitive circulatory loop undergoes sprouting and branching via a two-step navigation mechanism to form the trunk vascular network. Various studies using several model systems have uncovered a number of signaling mechanisms that regulate these complex processes. A genetic approach in zebrafish has led to identification of mutations and molecules that are responsible for specification of endothelial progenitor cells, differentiation of arterial and venous cells, and patterning of the dorsal aorta and intersegmental vessels. These studies highlight the unique utilities and benefits of the zebrafish system for studying development of embryonic blood vessels.