Ultrasensitive electrical detection of hepatitis b virus using silicon nanowire sensor

Anran Gao; Na Lu; Pengfei Dai; Tie Li; Yuelin Wang

Ultrasensitive electrical detection of hepatitis b virus using silicon nanowire sensor

Anran Gao
, Na Lu
, Pengfei Dai
, Tie Li^*
, Yuelin Wang

^*此作品的通讯作者

CAS - Shanghai Institute of Microsystem and Information Technology

科研成果: 书/报告/会议事项章节 › 会议稿件 › 同行评审

摘要

Herein, we describe a novel approach for rapid, label-free and ultrasensitive detection of Hepatitis B virus (HBV) DNA by applying rolling circle amplification (RCA) based on silicon nanowire field-effect transistor (SiNW-FET). Optical lithography and anisotropic self-stop etching were employed to guarantee low cost batch production for SiNWs. The RCA reaction created a long single-stranded DNA (ssDNA) product and thus enhanced the electronic responses of SiNW significantly. The ultrahigh sensitivity for 1 fM HBV DNA detection and high specificity for single-nucleotide polymorphism discrimination were achieved.

源语言	英语
主期刊名	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
出版商	Chemical and Biological Microsystems Society
页	2008-2010
页数	3
ISBN（电子版）	9780979806476
出版状态	已出版 - 2014
已对外发布	是
活动	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, 美国期限: 26 10月 2014 → 30 10月 2014

出版系列

姓名	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

会议

会议	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
国家/地区	美国
市	San Antonio
时期	26/10/14 → 30/10/14

联合国可持续发展目标

此成果有助于实现下列可持续发展目标：

可持续发展目标 3 良好健康与福祉

其它文件与链接

链接到 Scopus 的出版物

引用此

Gao, A., Lu, N., Dai, P., Li, T., & Wang, Y. (2014). Ultrasensitive electrical detection of hepatitis b virus using silicon nanowire sensor. 在 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (页码 2008-2010). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Gao, Anran ; Lu, Na ; Dai, Pengfei 等. / Ultrasensitive electrical detection of hepatitis b virus using silicon nanowire sensor. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. 页码 2008-2010 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

@inproceedings{a0df50240cb6442fa83ce65df575959d,

title = "Ultrasensitive electrical detection of hepatitis b virus using silicon nanowire sensor",

abstract = "Herein, we describe a novel approach for rapid, label-free and ultrasensitive detection of Hepatitis B virus (HBV) DNA by applying rolling circle amplification (RCA) based on silicon nanowire field-effect transistor (SiNW-FET). Optical lithography and anisotropic self-stop etching were employed to guarantee low cost batch production for SiNWs. The RCA reaction created a long single-stranded DNA (ssDNA) product and thus enhanced the electronic responses of SiNW significantly. The ultrahigh sensitivity for 1 fM HBV DNA detection and high specificity for single-nucleotide polymorphism discrimination were achieved.",

keywords = "CMOS-compatible, Nanosensor, RCA, Silicon nanowire",

author = "Anran Gao and Na Lu and Pengfei Dai and Tie Li and Yuelin Wang",

note = "Publisher Copyright: {\textcopyright} 14CBMS.; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",

year = "2014",

language = "英语",

series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

publisher = "Chemical and Biological Microsystems Society",

pages = "2008--2010",

booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

}

Gao, A, Lu, N, Dai, P, Li, T & Wang, Y 2014, Ultrasensitive electrical detection of hepatitis b virus using silicon nanowire sensor. 在 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, 页码 2008-2010, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, 美国, 26/10/14.

Ultrasensitive electrical detection of hepatitis b virus using silicon nanowire sensor. / Gao, Anran; Lu, Na; Dai, Pengfei 等.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. 页码 2008-2010 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

科研成果: 书/报告/会议事项章节 › 会议稿件 › 同行评审

TY - GEN

T1 - Ultrasensitive electrical detection of hepatitis b virus using silicon nanowire sensor

AU - Gao, Anran

AU - Lu, Na

AU - Dai, Pengfei

AU - Li, Tie

AU - Wang, Yuelin

PY - 2014

Y1 - 2014

N2 - Herein, we describe a novel approach for rapid, label-free and ultrasensitive detection of Hepatitis B virus (HBV) DNA by applying rolling circle amplification (RCA) based on silicon nanowire field-effect transistor (SiNW-FET). Optical lithography and anisotropic self-stop etching were employed to guarantee low cost batch production for SiNWs. The RCA reaction created a long single-stranded DNA (ssDNA) product and thus enhanced the electronic responses of SiNW significantly. The ultrahigh sensitivity for 1 fM HBV DNA detection and high specificity for single-nucleotide polymorphism discrimination were achieved.

AB - Herein, we describe a novel approach for rapid, label-free and ultrasensitive detection of Hepatitis B virus (HBV) DNA by applying rolling circle amplification (RCA) based on silicon nanowire field-effect transistor (SiNW-FET). Optical lithography and anisotropic self-stop etching were employed to guarantee low cost batch production for SiNWs. The RCA reaction created a long single-stranded DNA (ssDNA) product and thus enhanced the electronic responses of SiNW significantly. The ultrahigh sensitivity for 1 fM HBV DNA detection and high specificity for single-nucleotide polymorphism discrimination were achieved.

KW - CMOS-compatible

KW - Nanosensor

KW - RCA

KW - Silicon nanowire

UR - https://www.scopus.com/pages/publications/84941619320

M3 - 会议稿件

AN - SCOPUS:84941619320

T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

SP - 2008

EP - 2010

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Y2 - 26 October 2014 through 30 October 2014

ER -

Gao A, Lu N, Dai P, Li T, Wang Y. Ultrasensitive electrical detection of hepatitis b virus using silicon nanowire sensor. 在 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. 页码 2008-2010. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Ultrasensitive electrical detection of hepatitis b virus using silicon nanowire sensor

摘要

出版系列

会议

联合国可持续发展目标

其它文件与链接

指纹

引用此