The xyloglucan galactosylation modulates the cell wall stability of pollen tube

Main conclusion: A pollen specific homolog to a xyloglucan galactosyltransferase regulates cell wall stability and therefore pollen tube growth in Arabidopsis. Abstract: In angiosperms, pollen tubes grow through the transmitting tract to deliver the sperm cells to the ovule for fertilization. Fast growing pollen tubes coordinate the synthesis, secretion and assembly of cell wall components to maintain the mechanical properties of the cell wall. Xyloglucan, the major hemicellulosic polysaccharide in the primary cell wall, tethers cellulose to form the complexed cell wall network through its side chain modifications. How the side chain modifications of the xyloglucan regulate the pollen tube cell wall strength and growth remains elusive. Here we found that AtGT11, a MUR3 xyloglucan galactosyltransferase homolog highly expressed in pollen regulated the cell wall stability of pollen tubes. Genetic analysis of the gt11 and the xylosyltransferase 1/2 mutant indicated that the xylosylation of XyG side chains played dominant role while galactosylation of the XyG side chains finely modified the cell wall mechanics.