The influence of mesoporous bioactive glass on the IGF-II gene expression of osteoblasts

To investigate the bioactivity of mesoporous bioactive glass (MBG) and its effect on the IGF-II gene expression of rat osteoblast in vitro. Methods Rat osteoblasts were cultured with the ionic products of MBG dissolution in MEM. The cell activity and alkaline phosphatase (ALP) activity of rat osteoblasts were measured with MTT and PNPP. The mRNA expression of IGF- II was measured with RT-PCR. The concentration of IGF- II protein and IGFBP were measured with ELISA. Results After treatment by the MBG released ions, the ALP activity was increased to 126% of the control. The expression of IGF-II was increased to 126%. The concentration of unbound IGF-II protein and IGF bind protein (IGFBP) was increased to 175% and 237%, respectively. However, the cell activity was only 92% of the control. Conclusions The ionic products of MBG dissolution stimulate the gene expression of IGF- II and increase its protein synthesis, which is likely to be responsible for promoting the differentiation of osteoblast obviously, while the cell activity does not change markedly.