Synthesis of 7-Aminocoumarin by Buchwald-hartwig cross coupling for specific protein labeling in living cells

Xin Jin; Chayasith Uttamapinant; Alice Y. Ting

doi:10.1002/cbic.201000414

Synthesis of 7-Aminocoumarin by Buchwald-hartwig cross coupling for specific protein labeling in living cells

Xin Jin
, Chayasith Uttamapinant
, Alice Y. Ting^*

^*此作品的通讯作者

Massachusetts Institute of Technology

科研成果: 期刊稿件 › 文章 › 同行评审

45 引用（Scopus）

摘要

PRIME time: We report the synthesis of a pH-insensitive blue fluorophore, 7-aminocoumarin, by using palladium- catalyzed Buchwald-Hartwig cross coupling. 7-Aminocoumarin can be used to tag recombinant proteins on the cell surface and inside living cells through PRIME (probe incorporation mediated by enzymes), and unlike 7-hydroxycoumarin, can be visualized in acidic organelles such as endosomes.

源语言	英语
页（从-至）	65-70
页数	6
期刊	ChemBioChem
卷	12
期	1
DOI	https://doi.org/10.1002/cbic.201000414
出版状态	已出版 - 1月 2011
已对外发布	是

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abstract = "PRIME time: We report the synthesis of a pH-insensitive blue fluorophore, 7-aminocoumarin, by using palladium- catalyzed Buchwald-Hartwig cross coupling. 7-Aminocoumarin can be used to tag recombinant proteins on the cell surface and inside living cells through PRIME (probe incorporation mediated by enzymes), and unlike 7-hydroxycoumarin, can be visualized in acidic organelles such as endosomes.",

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year = "2011",

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AU - Uttamapinant, Chayasith

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N2 - PRIME time: We report the synthesis of a pH-insensitive blue fluorophore, 7-aminocoumarin, by using palladium- catalyzed Buchwald-Hartwig cross coupling. 7-Aminocoumarin can be used to tag recombinant proteins on the cell surface and inside living cells through PRIME (probe incorporation mediated by enzymes), and unlike 7-hydroxycoumarin, can be visualized in acidic organelles such as endosomes.

AB - PRIME time: We report the synthesis of a pH-insensitive blue fluorophore, 7-aminocoumarin, by using palladium- catalyzed Buchwald-Hartwig cross coupling. 7-Aminocoumarin can be used to tag recombinant proteins on the cell surface and inside living cells through PRIME (probe incorporation mediated by enzymes), and unlike 7-hydroxycoumarin, can be visualized in acidic organelles such as endosomes.

KW - Cell imaging

KW - Cross-coupling

KW - Enzyme engineering

KW - Fluorescent probes

KW - Palladium

UR - https://www.scopus.com/pages/publications/78650688480

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Synthesis of 7-Aminocoumarin by Buchwald-hartwig cross coupling for specific protein labeling in living cells

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