TY - JOUR
T1 - Surface‐enhanced Raman scattering technology based on TiO2/Nb2C coated microfluidic chip for monitoring glioma cells invasion in real time
AU - Zhao, Jie
AU - Liu, Xiaoyan
AU - Zhou, Yan
AU - Zheng, Tingting
AU - Tian, Yang
N1 - Publisher Copyright:
© 2023
PY - 2023/6
Y1 - 2023/6
N2 - Glioma is a malignant primary brain tumor that is extremely harmful to human beings. Therefore, studying the invasiveness of glioma cells is of great significance for the diagnosis and treatment of glioma. In this work, TiO2/Nb2C was prepared as a SERS substrate and combined with microfluidic chip to construct an invasion model capable of monitoring glioma invasion in real time. Both experimental data and density function theory (DFT) calculations showed that the significant SERS-enhancing effect of TiO2/Nb2C on methylene blue (MB) originated from the chemical magnification (CM) mechanism when MB was used as the adsorbed molecule. Based on this, we achieved a highly sensitive and targeted detection of vascular endothelial growth factor (VEGF), a biomarker for glioma with a low detection limit of 3.7 pg/mL, then quantified the invasive process in real time by detecting VEGF. Meanwhile, the depletion of reactive oxygen species (ROS) by TiO2/Nb2C can inhibit the invasion of glioma cells. For the first time, the invasion model combines SERS technology with microfluidic technology, while monitoring the cell invasion process in real time, the invasion process can be quantified by detecting the VEGF secreted by glioma cells during the invasion process, realizing the integration of diagnosis and treatment, and establish a new model for the biomedical analysis, clinical diagnosis and treatment of glioma.
AB - Glioma is a malignant primary brain tumor that is extremely harmful to human beings. Therefore, studying the invasiveness of glioma cells is of great significance for the diagnosis and treatment of glioma. In this work, TiO2/Nb2C was prepared as a SERS substrate and combined with microfluidic chip to construct an invasion model capable of monitoring glioma invasion in real time. Both experimental data and density function theory (DFT) calculations showed that the significant SERS-enhancing effect of TiO2/Nb2C on methylene blue (MB) originated from the chemical magnification (CM) mechanism when MB was used as the adsorbed molecule. Based on this, we achieved a highly sensitive and targeted detection of vascular endothelial growth factor (VEGF), a biomarker for glioma with a low detection limit of 3.7 pg/mL, then quantified the invasive process in real time by detecting VEGF. Meanwhile, the depletion of reactive oxygen species (ROS) by TiO2/Nb2C can inhibit the invasion of glioma cells. For the first time, the invasion model combines SERS technology with microfluidic technology, while monitoring the cell invasion process in real time, the invasion process can be quantified by detecting the VEGF secreted by glioma cells during the invasion process, realizing the integration of diagnosis and treatment, and establish a new model for the biomedical analysis, clinical diagnosis and treatment of glioma.
KW - Glioma cells
KW - Integration of diagnosis and treatment
KW - Microfluidic chips
KW - Surface-enhanced Raman spectroscopy
KW - VEGF
UR - https://www.scopus.com/pages/publications/85151838783
U2 - 10.1016/j.cclet.2022.107895
DO - 10.1016/j.cclet.2022.107895
M3 - 文章
AN - SCOPUS:85151838783
SN - 1001-8417
VL - 34
JO - Chinese Chemical Letters
JF - Chinese Chemical Letters
IS - 6
M1 - 107895
ER -