Study on microscope hyperspectral medical imaging method for biomedical quantitative analysis

A microscopic pushbroom hyperspectral imaging system was developed based on the microscopic technology and spectral imaging technology according to the principle of spectral imager in remote sensing. The basic principle and key technologies of this system were presented and the system performance was also analyzed. Some methods and algorithms were proposed to preprocess and normalize the microscopic hyperspectral data and retrieve the transmittance spectrum of samples. As a case study, the microscopic hyperspectral imaging system was used to image the retina sections of different rats and get some significant results. Experiment results show that the system can be used for the quantitative assessment and evaluating the effect of medication in biomedical research.