TY - JOUR
T1 - Study of pharmacokinetics and tissue distribution of norvancomycin in rats by CE with electrochemical detection
AU - Zhou, Tianshu
AU - Wu, Fang
AU - Shi, Guoyue
AU - Wang, Aifang
AU - Hu, Qin
AU - Wang, Xiaochun
AU - Fang, Yuzhi
PY - 2006/5
Y1 - 2006/5
N2 - In this paper, we developed a sensitive and simple method to study the pharmacokinetics and tissue distribution of norvancomycin (NVCM) in experimental animals by using CE with electrochemical detection. Pharmacokinetics investigation was performed by the collection of blood samples at timed intervals following administration of NVCM. Pharmacokinetic parameters were calculated by the 3P87 pharmacokinetic program. The elimination half-life of NVCM was 42.4742 min with a clearance rate of 0.0233 mL. kg-1 - min-1. Additionally, drug distribution was studied by measuring the NVCM levels in kidney, lung, stomach, intestine, spleen, heart, liver, and cerebrum. Electrophoresis conditions such as buffer solution, working potential, separation voltage, and sampling time were also discussed. The linear range was from 0.8 to 540 μg/mL with a correlation coefficient of 0.9991. The detection limit was 0.3 μg/mL. This method was for the first time applied to study the pharmacokinetics and tissue distributions of NVCM in experimental animals.
AB - In this paper, we developed a sensitive and simple method to study the pharmacokinetics and tissue distribution of norvancomycin (NVCM) in experimental animals by using CE with electrochemical detection. Pharmacokinetics investigation was performed by the collection of blood samples at timed intervals following administration of NVCM. Pharmacokinetic parameters were calculated by the 3P87 pharmacokinetic program. The elimination half-life of NVCM was 42.4742 min with a clearance rate of 0.0233 mL. kg-1 - min-1. Additionally, drug distribution was studied by measuring the NVCM levels in kidney, lung, stomach, intestine, spleen, heart, liver, and cerebrum. Electrophoresis conditions such as buffer solution, working potential, separation voltage, and sampling time were also discussed. The linear range was from 0.8 to 540 μg/mL with a correlation coefficient of 0.9991. The detection limit was 0.3 μg/mL. This method was for the first time applied to study the pharmacokinetics and tissue distributions of NVCM in experimental animals.
KW - Capillary electrophoresis
KW - Electrochemical detection
KW - Norvancomycin
KW - Pharmacokinetics
UR - https://www.scopus.com/pages/publications/33646548635
U2 - 10.1002/elps.200400774
DO - 10.1002/elps.200400774
M3 - 文章
C2 - 16645943
AN - SCOPUS:33646548635
SN - 0173-0835
VL - 27
SP - 1790
EP - 1796
JO - Electrophoresis
JF - Electrophoresis
IS - 9
ER -