Stimulation by melittin of Na+-Ca2+ exchange current in ventricular myocytes of guinea pigs

Xue Mei Zhang; Shen Yang; Yi Zhang; Ping Zheng; Ming Hua Jiang; Zhao Nian Zhou

Stimulation by melittin of Na⁺-Ca²⁺ exchange current in ventricular myocytes of guinea pigs

Xue Mei Zhang
, Shen Yang^*
, Yi Zhang
, Ping Zheng
, Ming Hua Jiang
, Zhao Nian Zhou

^*此作品的通讯作者

Fudan University
Shanghai Institute of Physiology

科研成果: 期刊稿件 › 文章 › 同行评审

4 引用（Scopus）

摘要

AIM: To study the mechanism of calcium overload induced by melittin in myocytes. METHODS: Whole-cell patch-clamp technique was applied for recording the currents. RESULTS: Mel 0.05, 0.1 μmol/L increased the peak amplitude of I_Na (nA) from -2.1 ± 0.8 to -3.2±1.0 (n = 7, P < 0.05) and -3.7±1.5 (n = 7, P < 0.05) respectively at testing potential of -40 mV. Mel 0.05, 0.1, 0.2 μmol/L had no significant effect on I_Ca, but enhanced I_Na-Ca(pA) from 53±21 to 427±256 (n = 5, P< 0.05), 349±147 (n = 5, P < 0.01) and 320±97 (n = 5, P < 0.05) respectively at a testing potential of +50 mV. CONCLUSION: The stimulating effect of Mel on I_Na-Ca rather than the effect on I_Ca contributes to the calcium overload of myocytes.

源语言	英语
页（从-至）	10-14
页数	5
期刊	Zhongguo Yaoli Xuebao/Acta Pharmacologica Sinica
卷	22
期	1
出版状态	已出版 - 2001
已对外发布	是

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@article{d5b7a4ebdac94aadb923ab60fc1a772d,

title = "Stimulation by melittin of Na+-Ca2+ exchange current in ventricular myocytes of guinea pigs",

abstract = "AIM: To study the mechanism of calcium overload induced by melittin in myocytes. METHODS: Whole-cell patch-clamp technique was applied for recording the currents. RESULTS: Mel 0.05, 0.1 μmol/L increased the peak amplitude of INa (nA) from -2.1 ± 0.8 to -3.2±1.0 (n = 7, P < 0.05) and -3.7±1.5 (n = 7, P < 0.05) respectively at testing potential of -40 mV. Mel 0.05, 0.1, 0.2 μmol/L had no significant effect on ICa, but enhanced INa-Ca(pA) from 53±21 to 427±256 (n = 5, P< 0.05), 349±147 (n = 5, P < 0.01) and 320±97 (n = 5, P < 0.05) respectively at a testing potential of +50 mV. CONCLUSION: The stimulating effect of Mel on INa-Ca rather than the effect on ICa contributes to the calcium overload of myocytes.",

keywords = "Calcium channels, Melitten, Myocardium, Patch-clamp techniques, Sodium channels, Sodium-calcium exchanger",

author = "Zhang, \{Xue Mei\} and Shen Yang and Yi Zhang and Ping Zheng and Jiang, \{Ming Hua\} and Zhou, \{Zhao Nian\}",

year = "2001",

language = "英语",

volume = "22",

pages = "10--14",

journal = "Zhongguo Yaoli Xuebao/Acta Pharmacologica Sinica",

issn = "0253-9756",

publisher = "Shanghai Kexue Jishu Wenxian Chubanshe",

number = "1",

}

TY - JOUR

T1 - Stimulation by melittin of Na+-Ca2+ exchange current in ventricular myocytes of guinea pigs

AU - Zhang, Xue Mei

AU - Yang, Shen

AU - Zhang, Yi

AU - Zheng, Ping

AU - Jiang, Ming Hua

AU - Zhou, Zhao Nian

PY - 2001

Y1 - 2001

N2 - AIM: To study the mechanism of calcium overload induced by melittin in myocytes. METHODS: Whole-cell patch-clamp technique was applied for recording the currents. RESULTS: Mel 0.05, 0.1 μmol/L increased the peak amplitude of INa (nA) from -2.1 ± 0.8 to -3.2±1.0 (n = 7, P < 0.05) and -3.7±1.5 (n = 7, P < 0.05) respectively at testing potential of -40 mV. Mel 0.05, 0.1, 0.2 μmol/L had no significant effect on ICa, but enhanced INa-Ca(pA) from 53±21 to 427±256 (n = 5, P< 0.05), 349±147 (n = 5, P < 0.01) and 320±97 (n = 5, P < 0.05) respectively at a testing potential of +50 mV. CONCLUSION: The stimulating effect of Mel on INa-Ca rather than the effect on ICa contributes to the calcium overload of myocytes.

AB - AIM: To study the mechanism of calcium overload induced by melittin in myocytes. METHODS: Whole-cell patch-clamp technique was applied for recording the currents. RESULTS: Mel 0.05, 0.1 μmol/L increased the peak amplitude of INa (nA) from -2.1 ± 0.8 to -3.2±1.0 (n = 7, P < 0.05) and -3.7±1.5 (n = 7, P < 0.05) respectively at testing potential of -40 mV. Mel 0.05, 0.1, 0.2 μmol/L had no significant effect on ICa, but enhanced INa-Ca(pA) from 53±21 to 427±256 (n = 5, P< 0.05), 349±147 (n = 5, P < 0.01) and 320±97 (n = 5, P < 0.05) respectively at a testing potential of +50 mV. CONCLUSION: The stimulating effect of Mel on INa-Ca rather than the effect on ICa contributes to the calcium overload of myocytes.

KW - Calcium channels

KW - Melitten

KW - Myocardium

KW - Patch-clamp techniques

KW - Sodium channels

KW - Sodium-calcium exchanger

UR - https://www.scopus.com/pages/publications/0035134156

M3 - 文章

C2 - 11730554

AN - SCOPUS:0035134156

SN - 0253-9756

VL - 22

SP - 10

EP - 14

JO - Zhongguo Yaoli Xuebao/Acta Pharmacologica Sinica

JF - Zhongguo Yaoli Xuebao/Acta Pharmacologica Sinica

IS - 1

ER -

Stimulation by melittin of Na+-Ca2+ exchange current in ventricular myocytes of guinea pigs

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Stimulation by melittin of Na⁺-Ca²⁺ exchange current in ventricular myocytes of guinea pigs