TY - JOUR
T1 - Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor
AU - Li, Yuanting
AU - Li, Dawei
AU - Song, Wei
AU - Li, Meng
AU - Zou, Jie
AU - Long, Yitao
PY - 2012/12
Y1 - 2012/12
N2 - A disposable biosensor was fabricated using single-walled carbon nanotubes, gold nanoparticles and tyrosinase (SWCNTs-AuNPs-Tyr) modified screen-printed electrodes. The prepared biosensor was applied to the rapid determination of phenolic contaminants within 15 minutes. The SWCNTs-AuNPs-Tyr bionanocomposite sensing layer was characterized with scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry methods. The characterization results revealed that SWCNTs could lead to a high loading of tyrosinase (Tyr) with the large surface area and the porous morphology, while AuNPs could retain the bioactivity of Tyr and enhance the sensitivity. The detection conditions, including working potential, pH of supporting electrolyte and the amount of Tyr were optimumed. As an example, the biosensor for catechol determination displayed a linear range of 8. 0 × 10-8 to 2. 0 × 10-5 mol·L-1 with a detection limit of 4. 5 × 10-8 mol·L-1 (S/N = 3). This method has a rapid response time within 10 s, and shows excellent repeatability and stability. Moreover, the resulting biosensor could be disposable, low-cost, reliable and easy to carry. This kind of new Tyr biosensor provides great potential for rapid, on-site and cost-effective analysis of phenolic contaminants in environmental water samples.
AB - A disposable biosensor was fabricated using single-walled carbon nanotubes, gold nanoparticles and tyrosinase (SWCNTs-AuNPs-Tyr) modified screen-printed electrodes. The prepared biosensor was applied to the rapid determination of phenolic contaminants within 15 minutes. The SWCNTs-AuNPs-Tyr bionanocomposite sensing layer was characterized with scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry methods. The characterization results revealed that SWCNTs could lead to a high loading of tyrosinase (Tyr) with the large surface area and the porous morphology, while AuNPs could retain the bioactivity of Tyr and enhance the sensitivity. The detection conditions, including working potential, pH of supporting electrolyte and the amount of Tyr were optimumed. As an example, the biosensor for catechol determination displayed a linear range of 8. 0 × 10-8 to 2. 0 × 10-5 mol·L-1 with a detection limit of 4. 5 × 10-8 mol·L-1 (S/N = 3). This method has a rapid response time within 10 s, and shows excellent repeatability and stability. Moreover, the resulting biosensor could be disposable, low-cost, reliable and easy to carry. This kind of new Tyr biosensor provides great potential for rapid, on-site and cost-effective analysis of phenolic contaminants in environmental water samples.
KW - gold nanoparticles
KW - on-site determination
KW - phenolic contaminants
KW - screen-printed electrodes
KW - single-walled carbon nanotubes
KW - tyrosinase biosensor
UR - https://www.scopus.com/pages/publications/84870388396
U2 - 10.1007/s11783-012-0393-z
DO - 10.1007/s11783-012-0393-z
M3 - 文章
AN - SCOPUS:84870388396
SN - 1673-7415
VL - 6
SP - 831
EP - 838
JO - Frontiers of Environmental Science and Engineering in China
JF - Frontiers of Environmental Science and Engineering in China
IS - 6
ER -