Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels

Bella Shlyahovsky; Di Li; Eugenii Katz; Itamar Willner

doi:10.1016/j.bios.2006.10.009

Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels

Bella Shlyahovsky
, Di Li
, Eugenii Katz
, Itamar Willner^*

^*此作品的通讯作者

Hebrew University of Jerusalem

科研成果: 期刊稿件 › 文章 › 同行评审

102 引用（Scopus）

摘要

Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H₂O₂, and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H₂O₂.

源语言	英语
页（从-至）	2570-2576
页数	7
期刊	Biosensors and Bioelectronics
卷	22
期	11
DOI	https://doi.org/10.1016/j.bios.2006.10.009
出版状态	已出版 - 15 5月 2007
已对外发布	是

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title = "Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels",

abstract = "Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H2O2, and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H2O2.",

keywords = "Aptamer, Biosensor, Chemiluminescence, DNAzyme, Glucose oxidase, Thrombin",

author = "Bella Shlyahovsky and Di Li and Eugenii Katz and Itamar Willner",

year = "2007",

month = may,

day = "15",

doi = "10.1016/j.bios.2006.10.009",

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TY - JOUR

T1 - Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels

AU - Shlyahovsky, Bella

AU - Li, Di

AU - Katz, Eugenii

AU - Willner, Itamar

PY - 2007/5/15

Y1 - 2007/5/15

N2 - Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H2O2, and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H2O2.

AB - Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H2O2, and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H2O2.

KW - Aptamer

KW - Biosensor

KW - Chemiluminescence

KW - DNAzyme

KW - Glucose oxidase

KW - Thrombin

UR - https://www.scopus.com/pages/publications/33947599087

U2 - 10.1016/j.bios.2006.10.009

DO - 10.1016/j.bios.2006.10.009

M3 - 文章

C2 - 17098412

AN - SCOPUS:33947599087

SN - 0956-5663

VL - 22

SP - 2570

EP - 2576

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 11

ER -

Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels

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