TY - JOUR
T1 - Photolarvicidal effect of thienyl 1,3,4-thia(oxa)diazoles and their potential DNA photocleavage
AU - Huang, Qingchun
AU - Liu, Ligang
AU - Xiao, Ciying
AU - Xu, Yufang
AU - Qian, Xuhong
PY - 2004/6
Y1 - 2004/6
N2 - The photolarvicidal activities and DNA photocleavage effects of 10 thienyl 1,3,4-thia(oxa)diazoles were investigated. The compounds, as novel photosensitizers, showed strong larvicidal activities against Pseudaletia separata only under irradiated conditions. Thiadiazole containing Br atom (I2) exhibited the highest photolarvicidal activity with mortality about 70.7%. The degree of mortality was increased with increasing accessibility period of the insects to photosensitizer-loaded leaves during exposure to light. The compounds also exhibited considerable DNA photocleavage activities on agarose gel electrophoresis that scissored supercoiled pBR322 DNA into two bands, scDNA and ocDNA. Oxadiazole containing Br atom (II2) nicked pBR322 DNA to produce ocDNA with highest conversion about 76.21%. DTT decreased DNA photocleavage effects, but SOD increased photoactivities, in vitro. Br was important in improving the biofunctioning of the photosensitizers. These results demonstrate the potential mode of action of thienyl-thia(oxa)diazole photosensitizers as insecticidal agents.
AB - The photolarvicidal activities and DNA photocleavage effects of 10 thienyl 1,3,4-thia(oxa)diazoles were investigated. The compounds, as novel photosensitizers, showed strong larvicidal activities against Pseudaletia separata only under irradiated conditions. Thiadiazole containing Br atom (I2) exhibited the highest photolarvicidal activity with mortality about 70.7%. The degree of mortality was increased with increasing accessibility period of the insects to photosensitizer-loaded leaves during exposure to light. The compounds also exhibited considerable DNA photocleavage activities on agarose gel electrophoresis that scissored supercoiled pBR322 DNA into two bands, scDNA and ocDNA. Oxadiazole containing Br atom (II2) nicked pBR322 DNA to produce ocDNA with highest conversion about 76.21%. DTT decreased DNA photocleavage effects, but SOD increased photoactivities, in vitro. Br was important in improving the biofunctioning of the photosensitizers. These results demonstrate the potential mode of action of thienyl-thia(oxa)diazole photosensitizers as insecticidal agents.
KW - DNA photocleavage
KW - Photoactivated larvicidal activity
KW - Thienyl 1,3,4-thia(oxa)diazoles
UR - https://www.scopus.com/pages/publications/2942525756
U2 - 10.1016/j.pestbp.2004.03.001
DO - 10.1016/j.pestbp.2004.03.001
M3 - 文章
AN - SCOPUS:2942525756
SN - 0048-3575
VL - 79
SP - 42
EP - 48
JO - Pesticide Biochemistry and Physiology
JF - Pesticide Biochemistry and Physiology
IS - 2
ER -