One-Step Synthesis of Water-Soluble MoS₂ Quantum Dots via a Hydrothermal Method as a Fluorescent Probe for Hyaluronidase Detection

In this work, a bottom-up strategy is developed to synthesize water-soluble molybdenum disulfide quantum dots (MoS₂ QDs) through a simple, one-step hydrothermal method using ammonium tetrathiomolybdate [(NH₄)₂MoS₄] as the precursor and hydrazine hydrate as the reducing agent. The as-synthesized MoS₂ QDs are few-layered with a narrow size distribution, and the average diameter is about 2.8 nm. The resultant QDs show excitation-dependent blue fluorescence due to the polydispersity of the QDs. Moreover, the fluorescence can be quenched by hyaluronic acid (HA)-functionalized gold nanoparticles through a photoinduced electron-transfer mechanism. Hyaluronidase (HAase), an endoglucosidase, can cleave HA into proangiogenic fragments and lead to the aggregation of gold nanoparticles. As a result, the electron transfer is blocked and fluorescence is recovered. On the basis of this principle, a novel fluorescence sensor for HAase is developed with a linear range from 1 to 50 U/mL and a detection limit of 0.7 U/mL.