One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing

Cuiling Zhang; Caiping Ding; Guohua Zhou; Qin Xue; Yuezhong Xian

doi:10.1016/j.aca.2016.12.024

One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing

Cuiling Zhang
, Caiping Ding
, Guohua Zhou
, Qin Xue
, Yuezhong Xian^*

^*此作品的通讯作者

化学与分子工程学院

East China Normal University
Lingnan Normal University

科研成果: 期刊稿件 › 文章 › 同行评审

15 引用（Scopus）

摘要

DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn²⁺) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS₂ nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn²⁺ QDs, but also provides a platform for the investigation of small molecule-protein interaction.

源语言	英语
页（从-至）	63-69
页数	7
期刊	Analytica Chimica Acta
卷	957
DOI	https://doi.org/10.1016/j.aca.2016.12.024
出版状态	已出版 - 8 3月 2017

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10.1016/j.aca.2016.12.024

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title = "One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing",

abstract = "DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn2+) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn2+ QDs, but also provides a platform for the investigation of small molecule-protein interaction.",

keywords = "Biosensor, FRET, Quantum dots, Terminal protection of small-molecule linked DNA, WS",

author = "Cuiling Zhang and Caiping Ding and Guohua Zhou and Qin Xue and Yuezhong Xian",

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doi = "10.1016/j.aca.2016.12.024",

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T1 - One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing

AU - Zhang, Cuiling

AU - Ding, Caiping

AU - Zhou, Guohua

AU - Xue, Qin

AU - Xian, Yuezhong

PY - 2017/3/8

Y1 - 2017/3/8

N2 - DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn2+) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn2+ QDs, but also provides a platform for the investigation of small molecule-protein interaction.

AB - DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn2+) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn2+ QDs, but also provides a platform for the investigation of small molecule-protein interaction.

KW - Biosensor

KW - FRET

KW - Quantum dots

KW - Terminal protection of small-molecule linked DNA

KW - WS

UR - https://www.scopus.com/pages/publications/85009371987

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DO - 10.1016/j.aca.2016.12.024

M3 - 文章

C2 - 28107835

AN - SCOPUS:85009371987

SN - 0003-2670

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SP - 63

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JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

ER -

One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing

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