"molecular beacon"-hosted thioflavin T: Applications for label-free fluorescent detection of iodide and logic operations

Yan Yun Li; Xiao Qin Jiang; Ling Fei Lu; Min Zhang; Guoyue Shi

doi:10.1016/j.talanta.2016.01.003

"molecular beacon"-hosted thioflavin T: Applications for label-free fluorescent detection of iodide and logic operations

Yan Yun Li
, Xiao Qin Jiang
, Ling Fei Lu
, Min Zhang^*
, Guoyue Shi

^*此作品的通讯作者

化学与分子工程学院

East China Normal University

科研成果: 期刊稿件 › 文章 › 同行评审

10 引用（Scopus）

摘要

In this work, we presented a simple, label-free and rapid-responsive fluorescence assay for iodide (I^-) detection based on "molecular beacon (MB)"-hosted thioflavin T (ThT), achieving a limit of detection as low as 158 nM. The proposed method exhibited very good selectivity to I^- ions over other anions interference due to the strong binding force between I^- ions with Hg²⁺. Upon the addition of I^- ions, it would capture Hg²⁺ from a T-Hg²⁺-T complex belonging to the MB-like DNA hairpin structure, which eventually quenched the initial fluorescence as output. In addition, it was successfully applied for operation of an integrated DNA logic gate system and to the determination of I^- in real samples such as human urine.

源语言	英语
页（从-至）	615-621
页数	7
期刊	Talanta
卷	150
DOI	https://doi.org/10.1016/j.talanta.2016.01.003
出版状态	已出版 - 1 4月 2016

访问文件

10.1016/j.talanta.2016.01.003

其它文件与链接

链接到 Scopus 的出版物

引用此

@article{44813b9c3f75462d8b6393aaf444ca3f,

title = "{"}molecular beacon{"}-hosted thioflavin T: Applications for label-free fluorescent detection of iodide and logic operations",

abstract = "In this work, we presented a simple, label-free and rapid-responsive fluorescence assay for iodide (I-) detection based on {"}molecular beacon (MB){"}-hosted thioflavin T (ThT), achieving a limit of detection as low as 158 nM. The proposed method exhibited very good selectivity to I- ions over other anions interference due to the strong binding force between I- ions with Hg2+. Upon the addition of I- ions, it would capture Hg2+ from a T-Hg2+-T complex belonging to the MB-like DNA hairpin structure, which eventually quenched the initial fluorescence as output. In addition, it was successfully applied for operation of an integrated DNA logic gate system and to the determination of I- in real samples such as human urine.",

keywords = "DNA, Iodide, Logic gate, Molecular beacon, Thioflavin T",

author = "Li, \{Yan Yun\} and Jiang, \{Xiao Qin\} and Lu, \{Ling Fei\} and Min Zhang and Guoyue Shi",

year = "2016",

month = apr,

day = "1",

doi = "10.1016/j.talanta.2016.01.003",

language = "英语",

volume = "150",

pages = "615--621",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - "molecular beacon"-hosted thioflavin T

T2 - Applications for label-free fluorescent detection of iodide and logic operations

AU - Li, Yan Yun

AU - Jiang, Xiao Qin

AU - Lu, Ling Fei

AU - Zhang, Min

AU - Shi, Guoyue

PY - 2016/4/1

Y1 - 2016/4/1

N2 - In this work, we presented a simple, label-free and rapid-responsive fluorescence assay for iodide (I-) detection based on "molecular beacon (MB)"-hosted thioflavin T (ThT), achieving a limit of detection as low as 158 nM. The proposed method exhibited very good selectivity to I- ions over other anions interference due to the strong binding force between I- ions with Hg2+. Upon the addition of I- ions, it would capture Hg2+ from a T-Hg2+-T complex belonging to the MB-like DNA hairpin structure, which eventually quenched the initial fluorescence as output. In addition, it was successfully applied for operation of an integrated DNA logic gate system and to the determination of I- in real samples such as human urine.

AB - In this work, we presented a simple, label-free and rapid-responsive fluorescence assay for iodide (I-) detection based on "molecular beacon (MB)"-hosted thioflavin T (ThT), achieving a limit of detection as low as 158 nM. The proposed method exhibited very good selectivity to I- ions over other anions interference due to the strong binding force between I- ions with Hg2+. Upon the addition of I- ions, it would capture Hg2+ from a T-Hg2+-T complex belonging to the MB-like DNA hairpin structure, which eventually quenched the initial fluorescence as output. In addition, it was successfully applied for operation of an integrated DNA logic gate system and to the determination of I- in real samples such as human urine.

KW - DNA

KW - Iodide

KW - Logic gate

KW - Molecular beacon

KW - Thioflavin T

UR - https://www.scopus.com/pages/publications/84953923867

U2 - 10.1016/j.talanta.2016.01.003

DO - 10.1016/j.talanta.2016.01.003

M3 - 文章

C2 - 26838450

AN - SCOPUS:84953923867

SN - 0039-9140

VL - 150

SP - 615

EP - 621

JO - Talanta

JF - Talanta

ER -

"molecular beacon"-hosted thioflavin T: Applications for label-free fluorescent detection of iodide and logic operations

摘要

访问文件

其它文件与链接

指纹

引用此