TY - JOUR
T1 - Metagenomic profiles of antibiotic resistance genes in paddy soils from South China
AU - Xiao, Ke Qing
AU - Li, Bing
AU - Ma, Liping
AU - Bao, Peng
AU - Zhou, Xue
AU - Zhang, Tong
AU - Zhu, Yong Guan
N1 - Publisher Copyright:
© FEMS 2016.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Overuse and arbitrary discarding of antibiotics have expanded antibiotic resistance reservoirs, from gut, waste water and activated sludge, to soil, freshwater and even the ocean. Based on the structured Antibiotic Resistance Genes Database and next generation sequencing, metagenomic analysis was used for the first time to detect and quantify antibiotic resistance genes (ARGs) in paddy soils from South China. A total of 16 types of ARGs were identified, corresponding to 110 ARG subtypes. The abundances and distribution pattern of ARGs in paddy soil were distinctively different from those in activated sludge and pristine deep ocean sediment, but close to those of sediment from human-impacted estuaries. Multidrug resistance genes were the most dominant type (38-47.5%) in all samples, and the ARGs detected encompassed the three major resistance mechanisms, among which extrusion by efflux pumps was predominant. Redundancy analysis (RDA) showed that pH was significantly correlated with the distribution of ARG subtypes (P < 0.05). Our results provided a broad spectrum profile of ARGs in paddy soil, indicating that ARGs are widespread in paddy soils of South China.
AB - Overuse and arbitrary discarding of antibiotics have expanded antibiotic resistance reservoirs, from gut, waste water and activated sludge, to soil, freshwater and even the ocean. Based on the structured Antibiotic Resistance Genes Database and next generation sequencing, metagenomic analysis was used for the first time to detect and quantify antibiotic resistance genes (ARGs) in paddy soils from South China. A total of 16 types of ARGs were identified, corresponding to 110 ARG subtypes. The abundances and distribution pattern of ARGs in paddy soil were distinctively different from those in activated sludge and pristine deep ocean sediment, but close to those of sediment from human-impacted estuaries. Multidrug resistance genes were the most dominant type (38-47.5%) in all samples, and the ARGs detected encompassed the three major resistance mechanisms, among which extrusion by efflux pumps was predominant. Redundancy analysis (RDA) showed that pH was significantly correlated with the distribution of ARG subtypes (P < 0.05). Our results provided a broad spectrum profile of ARGs in paddy soil, indicating that ARGs are widespread in paddy soils of South China.
KW - ARGs
KW - Metagenomic analysis
KW - Paddy soil
UR - https://www.scopus.com/pages/publications/84962194814
U2 - 10.1093/femsec/fiw023
DO - 10.1093/femsec/fiw023
M3 - 文章
C2 - 26850156
AN - SCOPUS:84962194814
SN - 0168-6496
VL - 92
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
IS - 3
ER -