Liquid chromatography-electrochemical detection for studying the effects of tetrahydrobiopterin on monoamine neurotransmitters in rat striatum

Tetrahydrobiopterin (BH ₄ ) is an essential co-factor in the biosynthesis of monoamine neurotransmitters. A nano-Pt/Pd modified electrode as the electrochemical detector (ED) for high-performance liquid chromatography (HPLC) coupled with microanalysis sampling, is used to explore the effect of administration of BH ₄ on the monoamine neurotransmitters in the rat striatum. The researches demonstrate that the contents of dopamine (DA), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA) increase significantly with the administration of BH ₄ . The pharmaceutical kinetics is carried out to research into the time course of BH ₄ effect on the concentration of monoamine neurotransmitters in rat striatum, which provides reliable data for pathology and pharmacology research on neuroscience.