TY - JOUR
T1 - Label-free detection of DNA hybridization based on a novel functionalized conducting polymer
AU - Peng, Hui
AU - Soeller, Christian
AU - Vigar, Nicholas A.
AU - Caprio, Vittorio
AU - Travas-Sejdic, Jadranka
PY - 2007/4/15
Y1 - 2007/4/15
N2 - A new functionalized pyrrole monomer, 3-pyrrolylacrylic acid (PAA) was synthesized. It was used to prepare a copolymer with pyrrole, poly(Py-co-PAA), which was investigated by reflective FT-IR, UV-vis spectroscopy and cyclic voltammetry. A label-free DNA sensor was prepared based on a poly(Py-co-PAA) film. Hybridization with complementary and non-complementary DNA targets was studied by electrochemical impedance spectroscopy. Results show a significant increase in the charge-transfer resistance upon addition of complementary target. The impedance spectra were analyzed by using a modified Randles and Ershler equivalent circuit model. The change in charge-transfer resistance that was used as an index of sensor response was found to be linear with logarithmic target concentration in the range of 2 × 10-9 to 2 × 10-7 M. The detection limit was 0.98 nM.
AB - A new functionalized pyrrole monomer, 3-pyrrolylacrylic acid (PAA) was synthesized. It was used to prepare a copolymer with pyrrole, poly(Py-co-PAA), which was investigated by reflective FT-IR, UV-vis spectroscopy and cyclic voltammetry. A label-free DNA sensor was prepared based on a poly(Py-co-PAA) film. Hybridization with complementary and non-complementary DNA targets was studied by electrochemical impedance spectroscopy. Results show a significant increase in the charge-transfer resistance upon addition of complementary target. The impedance spectra were analyzed by using a modified Randles and Ershler equivalent circuit model. The change in charge-transfer resistance that was used as an index of sensor response was found to be linear with logarithmic target concentration in the range of 2 × 10-9 to 2 × 10-7 M. The detection limit was 0.98 nM.
KW - Electrochemical impedance spectroscopy
KW - Functionalized polypyrrole
KW - Hybridization
KW - Label-free DNA detection
UR - https://www.scopus.com/pages/publications/33847417895
U2 - 10.1016/j.bios.2006.07.010
DO - 10.1016/j.bios.2006.07.010
M3 - 文章
C2 - 16919441
AN - SCOPUS:33847417895
SN - 0956-5663
VL - 22
SP - 1868
EP - 1873
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 9-10
ER -