TY - JOUR
T1 - Ion transport and metabolic regulation induced by nanoplastic toxicity in gill of Litopenaeus vannamei using proteomics
AU - Li, Yiming
AU - Ye, Yucong
AU - Zhu, Xiaoyi
AU - Yao, Zongli
AU - Li, Yan
AU - Sun, Zhen
AU - Rihan, Na
AU - Zhao, Yunlong
AU - Lai, Qifang
N1 - Publisher Copyright:
© 2025 The Royal Society of Chemistry.
PY - 2025/5/27
Y1 - 2025/5/27
N2 - Polystyrene nanoplastic (PS-NP) pollution has become a global environmental problem, and its potential toxicity to aquatic organisms has led to widespread concern. In this study, a 28-day chronic nanoplastic exposure (0, 0.1, 1, 5, and 10 mg L−1) experiment was established to investigate the effects of ion transport and metabolic regulation in Pacific white shrimp Litopenaeus vannamei. The results showed that the ion content in gill tissues decreased and the ATPase activities significantly decreased with the increase in PS-NP concentration. PS-NP exposure destroyed the gill tissue structure, disrupted the activity of ion transport enzymes, and affected the glutathione metabolism. The blood urea nitrogen, glutamate, and proline contents first increased and then decreased with the increase in PS-NP concentration. The expression of nitric oxide synthase and thioredoxin genes involved in energy metabolism decreased. The urea nitrogen content was negatively correlated with the ion transport module, and the proline content was positively correlated with the ion transport module and gene expression. Proteomic analysis results showed that PS-NP exposure mainly affected the amino acid biosynthesis and mTOR signaling pathway. These results suggested that PS-NP exposure negatively affected the physiological state of L. vannamei by interfering with the urea cycle and glutathione metabolism via inhibition of ion transport.
AB - Polystyrene nanoplastic (PS-NP) pollution has become a global environmental problem, and its potential toxicity to aquatic organisms has led to widespread concern. In this study, a 28-day chronic nanoplastic exposure (0, 0.1, 1, 5, and 10 mg L−1) experiment was established to investigate the effects of ion transport and metabolic regulation in Pacific white shrimp Litopenaeus vannamei. The results showed that the ion content in gill tissues decreased and the ATPase activities significantly decreased with the increase in PS-NP concentration. PS-NP exposure destroyed the gill tissue structure, disrupted the activity of ion transport enzymes, and affected the glutathione metabolism. The blood urea nitrogen, glutamate, and proline contents first increased and then decreased with the increase in PS-NP concentration. The expression of nitric oxide synthase and thioredoxin genes involved in energy metabolism decreased. The urea nitrogen content was negatively correlated with the ion transport module, and the proline content was positively correlated with the ion transport module and gene expression. Proteomic analysis results showed that PS-NP exposure mainly affected the amino acid biosynthesis and mTOR signaling pathway. These results suggested that PS-NP exposure negatively affected the physiological state of L. vannamei by interfering with the urea cycle and glutathione metabolism via inhibition of ion transport.
UR - https://www.scopus.com/pages/publications/105006781946
U2 - 10.1039/d5en00040h
DO - 10.1039/d5en00040h
M3 - 文章
AN - SCOPUS:105006781946
SN - 2051-8153
VL - 12
SP - 3592
EP - 3608
JO - Environmental Science: Nano
JF - Environmental Science: Nano
IS - 7
ER -