Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity

Min Zhang; Bin Cheng Yin; Xian Fei Wang; Bang Ce Ye

doi:10.1039/c0cc04887a

Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity

Min Zhang
, Bin Cheng Yin
, Xian Fei Wang
, Bang Ce Ye^*

^*此作品的通讯作者

East China University of Science and Technology

科研成果: 期刊稿件 › 文章 › 同行评审

223 引用（Scopus）

摘要

We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.

源语言	英语
页（从-至）	2399-2401
页数	3
期刊	Chemical Communications
卷	47
期	8
DOI	https://doi.org/10.1039/c0cc04887a
出版状态	已出版 - 7 2月 2011
已对外发布	是

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title = "Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity",

abstract = "We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.",

author = "Min Zhang and Yin, \{Bin Cheng\} and Wang, \{Xian Fei\} and Ye, \{Bang Ce\}",

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AU - Zhang, Min

AU - Yin, Bin Cheng

AU - Wang, Xian Fei

AU - Ye, Bang Ce

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N2 - We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.

AB - We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.

UR - https://www.scopus.com/pages/publications/79751518967

U2 - 10.1039/c0cc04887a

DO - 10.1039/c0cc04887a

M3 - 文章

C2 - 21305066

AN - SCOPUS:79751518967

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JO - Chemical Communications

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Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity

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