TY - JOUR
T1 - Hydroxylamine metabolism in mainstream denitrifying ammonium oxidation (DEAMOX) process
T2 - Achieving fast start-up and robust operation with bio-augmentation assistance under ambient temperature
AU - Zhang, Xingxing
AU - Zhang, Xiaonong
AU - Wu, Peng
AU - Ma, Liping
AU - Chen, Junjiang
AU - Wang, Chaochao
AU - Li, Xiang
AU - Liu, Wenru
AU - Xu, Lezhong
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/1/5
Y1 - 2022/1/5
N2 - Nitrogen removal from mainstream wastewater via DEnitrifying AMmonium OXidation (DEAMOX) is often challenged by undulated actual temperature and high loading rate. Here, we discovered NH2OH addition (HA) and bio-augmentation (BA) tactics on start-up and operation performance of DEAMOXs (R1 and R2) under ambient temperature (11.3–31.7 °C). Over 340-day operation suggested that R2 received 10 mg/L HA and 1:25 BA ratio (v/v, anammox/partial denitrification sludge) achieved desirable nitrogen removal efficiency (NRE) of 97.22% after 145-day, while R1 under higher BA ratio of 1:12.5 without HA obtained lower NRE (90.86%) after 184-day. Batch tests revealed that nitrate-nitrite transformation ratio reached 98.64% at low COD/NO3-–N of 2.6 with HA. Significantly, compared with R2, R1 recovered quickly with satisfactory effluent total nitrogen of 4.21 mg/L despite nitrogen loading rate greater than 0.15 kg N/m3/d and temperature decreased to 14.6 °C. The abundant narG represented high nitrate reduction potential, hzsA and hdh were extensively detected as the symbolisation of anammox metabolism. Thauera, Denitratisoma and unclassified f Comamonadaceae dominated nitrite accumulation. Ca. Brocadia as the dominant anammox bacteria, and its population maintained stable against low temperature and load shocks by NH2OH intensification. Overall, this study offers an opportunity for the wide-applications of DEAMOX treating mainstream wastewater.
AB - Nitrogen removal from mainstream wastewater via DEnitrifying AMmonium OXidation (DEAMOX) is often challenged by undulated actual temperature and high loading rate. Here, we discovered NH2OH addition (HA) and bio-augmentation (BA) tactics on start-up and operation performance of DEAMOXs (R1 and R2) under ambient temperature (11.3–31.7 °C). Over 340-day operation suggested that R2 received 10 mg/L HA and 1:25 BA ratio (v/v, anammox/partial denitrification sludge) achieved desirable nitrogen removal efficiency (NRE) of 97.22% after 145-day, while R1 under higher BA ratio of 1:12.5 without HA obtained lower NRE (90.86%) after 184-day. Batch tests revealed that nitrate-nitrite transformation ratio reached 98.64% at low COD/NO3-–N of 2.6 with HA. Significantly, compared with R2, R1 recovered quickly with satisfactory effluent total nitrogen of 4.21 mg/L despite nitrogen loading rate greater than 0.15 kg N/m3/d and temperature decreased to 14.6 °C. The abundant narG represented high nitrate reduction potential, hzsA and hdh were extensively detected as the symbolisation of anammox metabolism. Thauera, Denitratisoma and unclassified f Comamonadaceae dominated nitrite accumulation. Ca. Brocadia as the dominant anammox bacteria, and its population maintained stable against low temperature and load shocks by NH2OH intensification. Overall, this study offers an opportunity for the wide-applications of DEAMOX treating mainstream wastewater.
KW - Bioaugmented DEAMOX
KW - Biological nitrogen removal
KW - Fast start-up
KW - Hydroxylamine (NHOH) metabolism
KW - Nitrite accumulation
UR - https://www.scopus.com/pages/publications/85111292891
U2 - 10.1016/j.jhazmat.2021.126736
DO - 10.1016/j.jhazmat.2021.126736
M3 - 文章
C2 - 34333411
AN - SCOPUS:85111292891
SN - 0304-3894
VL - 421
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 126736
ER -