Highly selective fluorescent probe for vicinal-dithiol-containing proteins and in situ imaging in living cells

Chusen Huang; Qin Yin; Weiping Zhu; Yi Yang; Xin Wang; Xuhong Qian; Yufang Xu

doi:10.1002/anie.201101317

Highly selective fluorescent probe for vicinal-dithiol-containing proteins and in situ imaging in living cells

Chusen Huang^*
, Qin Yin
, Weiping Zhu
, Yi Yang
, Xin Wang
, Xuhong Qian
, Yufang Xu

^*此作品的通讯作者

East China University of Science and Technology

科研成果: 期刊稿件 › 文章 › 同行评审

88 引用（Scopus）

摘要

It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function.

源语言	英语
页（从-至）	7551-7556
页数	6
期刊	Angewandte Chemie - International Edition
卷	50
期	33
DOI	https://doi.org/10.1002/anie.201101317
出版状态	已出版 - 8 8月 2011
已对外发布	是

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abstract = "It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function.",

keywords = "fluorescent probes, in situ imaging, protein labeling, redox proteomics, vicinal dithiols",

author = "Chusen Huang and Qin Yin and Weiping Zhu and Yi Yang and Xin Wang and Xuhong Qian and Yufang Xu",

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AU - Huang, Chusen

AU - Yin, Qin

AU - Zhu, Weiping

AU - Yang, Yi

AU - Wang, Xin

AU - Qian, Xuhong

AU - Xu, Yufang

PY - 2011/8/8

Y1 - 2011/8/8

N2 - It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function.

AB - It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function.

KW - fluorescent probes

KW - in situ imaging

KW - protein labeling

KW - redox proteomics

KW - vicinal dithiols

UR - https://www.scopus.com/pages/publications/79961242017

U2 - 10.1002/anie.201101317

DO - 10.1002/anie.201101317

M3 - 文章

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AN - SCOPUS:79961242017

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EP - 7556

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 33

ER -

Highly selective fluorescent probe for vicinal-dithiol-containing proteins and in situ imaging in living cells

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