Higher immune response inflicted by combined immunization of HIV-2 gp105

Objective: To provide fundamental data for developing an effective immunization strategy by different types of recombinant vaccines to protect HIV-2 infection. Methods: Using prime-boost immunizations strategy, BALB/c mice were inoculated muscularly with HIV-2 gp105 recombinant DNA vaccine and fowlpox virus. The numbers of CD4⁺ and CD8⁺ subgroup of spleen T lymphocyte, the specific killing activities of spleen CTL and the titers of serum antibodies of the immunized mice were detected. Results: HIV-2 specific target-killing activity of spleen CTL and serum HIV-2 specific antibody level were higher in combined immunization group than those in single immunization groups (P<0.05). Conclusion: The stra tegy of prime-boost may stimulate stronger specific celluar and humoral immune responses in mice.