High performance liquid chromatography-electrochemical detection (HPLC-ECD) for the pharmacokinetic studies of acetaminophen with microdialysis

In this article, we combined microdialysis sampling with HPLC-ECD and studied the pharmacokinetics of acetaminophen. In the mobile phase of phosphate buffer solution (PBS, pH 7.0), the copper tetraaminophthalocyanine (CuTAPC) chemically modified electrode, which was used as working electrode, has good electrocatalytical oxidation of acetaminophen with a detection limit of 2.0 × 10^-7 mol/L and a linear concentration range of 6.0 × 10^-7 mol/L-1.0 × 10^-4 mol/L at +500 mV (vs. Ag/AgCl). In vivo microdialysis is an excellent complement to directly monitor chemical events in different organs and has been widely used for many fields. We applied this method to study the pharmacokinetic of acetaminophen (ACE). The estimated association constant (K) and the number of the binding sites (n) on one molecule of BSA were 5.37 × 10³ (mol/L)^-1 and 2.57, respectively. The calculated half-life of absorption was 33.0 ± 1.0 min, and that of elimination was 45.0 ± 2.0 min.