Generation of chemical-inducible activation tagging T-DNA insertion lines of Arabidopsis thaliana

Jian Zhang; Jin Xiang Xu; Ying Zhen Kong; Zhen Dong Ji; Xing Chun Wang; Feng Ying An; Chao Li; Jia Qiang Sun; Su Zhi Zhang; Xiao Hui Yang; Jin Ye Mu; Xin Fang Liu; Jia Yang Li; Yong Biao Xue; Jian Ru Zuo

Generation of chemical-inducible activation tagging T-DNA insertion lines of Arabidopsis thaliana

Jian Zhang^*
, Jin Xiang Xu
, Ying Zhen Kong
, Zhen Dong Ji
, Xing Chun Wang
, Feng Ying An
, Chao Li
, Jia Qiang Sun
, Su Zhi Zhang
, Xiao Hui Yang
, Jin Ye Mu
, Xin Fang Liu
, Jia Yang Li
, Yong Biao Xue
, Jian Ru Zuo

^*此作品的通讯作者

CAS - Institute of Genetics and Developmental Biology
University of Chinese Academy of Sciences

科研成果: 期刊稿件 › 文章 › 同行评审

24 引用（Scopus）

摘要

Using an estrogen-inducible expression XVE (LexA-VP16-Estragon Receptor) system, we have generated approximately 40 000 independent T-DNA insertion lines of Arabidopsis thaliana. Segregation analyses of about 18 000 lines indicated that 51.6% of them contain single T-DNA insertions and that the average insertion number is 1.38 copies per line. Mutants displaying a variety of morphological alterations were identified, including those that affect development of roots, hypocotyls, leaves, floral organs and seeds as well as the flowering time.

源语言	英语
页（从-至）	1082-1088
页数	7
期刊	Acta Genetica Sinica
卷	32
期	10
出版状态	已出版 - 10月 2005
已对外发布	是

其它文件与链接

链接到 Scopus 的出版物

引用此

@article{ea4e67a2d8644225b6c9cb9f33d35f3f,

title = "Generation of chemical-inducible activation tagging T-DNA insertion lines of Arabidopsis thaliana",

abstract = "Using an estrogen-inducible expression XVE (LexA-VP16-Estragon Receptor) system, we have generated approximately 40 000 independent T-DNA insertion lines of Arabidopsis thaliana. Segregation analyses of about 18 000 lines indicated that 51.6\% of them contain single T-DNA insertions and that the average insertion number is 1.38 copies per line. Mutants displaying a variety of morphological alterations were identified, including those that affect development of roots, hypocotyls, leaves, floral organs and seeds as well as the flowering time.",

keywords = "Arabidopsis thaliana, Inducible activation tagging, Mutants, T-DNA, XVE",

author = "Jian Zhang and Xu, \{Jin Xiang\} and Kong, \{Ying Zhen\} and Ji, \{Zhen Dong\} and Wang, \{Xing Chun\} and An, \{Feng Ying\} and Chao Li and Sun, \{Jia Qiang\} and Zhang, \{Su Zhi\} and Yang, \{Xiao Hui\} and Mu, \{Jin Ye\} and Liu, \{Xin Fang\} and Li, \{Jia Yang\} and Xue, \{Yong Biao\} and Zuo, \{Jian Ru\}",

year = "2005",

month = oct,

language = "英语",

volume = "32",

pages = "1082--1088",

journal = "Acta Genetica Sinica",

issn = "0379-4172",

publisher = "Institute of Genetics and Developmental Biology",

number = "10",

}

TY - JOUR

T1 - Generation of chemical-inducible activation tagging T-DNA insertion lines of Arabidopsis thaliana

AU - Zhang, Jian

AU - Xu, Jin Xiang

AU - Kong, Ying Zhen

AU - Ji, Zhen Dong

AU - Wang, Xing Chun

AU - An, Feng Ying

AU - Li, Chao

AU - Sun, Jia Qiang

AU - Zhang, Su Zhi

AU - Yang, Xiao Hui

AU - Mu, Jin Ye

AU - Liu, Xin Fang

AU - Li, Jia Yang

AU - Xue, Yong Biao

AU - Zuo, Jian Ru

PY - 2005/10

Y1 - 2005/10

N2 - Using an estrogen-inducible expression XVE (LexA-VP16-Estragon Receptor) system, we have generated approximately 40 000 independent T-DNA insertion lines of Arabidopsis thaliana. Segregation analyses of about 18 000 lines indicated that 51.6% of them contain single T-DNA insertions and that the average insertion number is 1.38 copies per line. Mutants displaying a variety of morphological alterations were identified, including those that affect development of roots, hypocotyls, leaves, floral organs and seeds as well as the flowering time.

AB - Using an estrogen-inducible expression XVE (LexA-VP16-Estragon Receptor) system, we have generated approximately 40 000 independent T-DNA insertion lines of Arabidopsis thaliana. Segregation analyses of about 18 000 lines indicated that 51.6% of them contain single T-DNA insertions and that the average insertion number is 1.38 copies per line. Mutants displaying a variety of morphological alterations were identified, including those that affect development of roots, hypocotyls, leaves, floral organs and seeds as well as the flowering time.

KW - Arabidopsis thaliana

KW - Inducible activation tagging

KW - Mutants

KW - T-DNA

KW - XVE

UR - https://www.scopus.com/pages/publications/26944495308

M3 - 文章

C2 - 16252704

AN - SCOPUS:26944495308

SN - 0379-4172

VL - 32

SP - 1082

EP - 1088

JO - Acta Genetica Sinica

JF - Acta Genetica Sinica

IS - 10

ER -

Generation of chemical-inducible activation tagging T-DNA insertion lines of Arabidopsis thaliana

摘要

其它文件与链接

指纹

引用此