Facile Synthesis of Leaf-Like CuO Nanoparticles and Their Application on Glucose Biosensor

An efficient amperometric biosensor based on well-crystallized leaf-like CuO nanoparticles for detecting glucose has been proposed. The leaf-like CuO nanoparticles, synthesized by a simple one-step hydrothermal method, were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscopy (TEM) for the morphology study. Under the optimal condition, the electrochemical behaviour of the leaf-like CuO nanoparticles modified electrode for detection of glucose exhibited high sensitivity of 246μA/mM/cm², short response time (within 5s), linear dynamic range from 1.0 to 170μM (R²=0.9995), and low limit of detection (LOD) (S/N=3) of 0.91μM. The high sensitivity, good reproducibility, stability, and fast amperometric sensing towards oxidation of glucose, make this biosensor promising for future application.