TY - JOUR
T1 - Excited-State Dynamics of Proflavine after Intercalation into DNA Duplex
AU - Zhou, Jie
AU - Jia, Yanyan
AU - Wang, Xueli
AU - Jia, Menghui
AU - Pan, Haifeng
AU - Sun, Zhenrong
AU - Chen, Jinquan
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2022/12
Y1 - 2022/12
N2 - Proflavine is an acridine derivative which was discovered as one of the earliest antibacterial agents, and it has been proven to have potential application to fields such as chemotherapy, photobiology and solar-energy conversion. In particular, it is well known that proflavine can bind to DNA with different modes, and this may open addition photochemical-reaction channels in DNA. Herein, the excited-state dynamics of proflavine after intercalation into DNA duplex is studied using femtosecond time-resolved spectroscopy, and compared with that in solution. It is demonstrated that both fluorescence and the triplet excited-state generation of proflavine were quenched after intercalation into DNA, due to ultrafast non-radiative channels. A static-quenching mechanism was identified for the proflavine-DNA complex, in line with the spectroscopy data, and the excited-state deactivation mechanism was proposed.
AB - Proflavine is an acridine derivative which was discovered as one of the earliest antibacterial agents, and it has been proven to have potential application to fields such as chemotherapy, photobiology and solar-energy conversion. In particular, it is well known that proflavine can bind to DNA with different modes, and this may open addition photochemical-reaction channels in DNA. Herein, the excited-state dynamics of proflavine after intercalation into DNA duplex is studied using femtosecond time-resolved spectroscopy, and compared with that in solution. It is demonstrated that both fluorescence and the triplet excited-state generation of proflavine were quenched after intercalation into DNA, due to ultrafast non-radiative channels. A static-quenching mechanism was identified for the proflavine-DNA complex, in line with the spectroscopy data, and the excited-state deactivation mechanism was proposed.
KW - DNA duplex
KW - excited-state dynamics
KW - femtosecond transient-absorption
KW - fluorescence-quenching
KW - proflavine
UR - https://www.scopus.com/pages/publications/85143625051
U2 - 10.3390/molecules27238157
DO - 10.3390/molecules27238157
M3 - 文章
C2 - 36500248
AN - SCOPUS:85143625051
SN - 1420-3049
VL - 27
JO - Molecules
JF - Molecules
IS - 23
M1 - 8157
ER -