Electrochemically renewable SERS sensor: A new platform for the detection of metabolites involved in peroxide production

The accurate detection of hydrogen peroxide (H₂O₂)-involved metabolites plays a significant role in the early diagnosis of metabolism-associated diseases, whereas most of current metabolite-sensing systems are often hindered by low sensitivity, interference of coexisting species, or tedious preparation. Herein, an electrochemistry-regenerated surface-enhanced Raman scattering (SERS) sensor was developed to serve as a universal platform for detecting H₂O₂-involved metabolites. The SERS sensor was constructed by modifying newly synthesized 2-mercaptohydroquinone (2-MHQ) molecules on the surface of gold nanoparticles (AuNPs) that were electrochemically predeposited on an ITO electrode. Metabolites were detected through the changes in the SERS spectrum as a result of the reaction of 2-MHQ with H₂O₂ induced by the metabolites. Combining the superiority of SERS fingerprint identification and the specificity of the related enzymatic reactions producing H₂O₂, the designed SERS sensor was highly selective in detecting glucose and uric acid as models of H₂O₂-involved metabolite with limits of detection (LODs) of 0.159 μM and 0.0857 μM, respectively. Moreover, the sensor maintained a high SERS activity even after more than 10 electrochemical regenerations within 2 min, demonstrating its effectiveness for the rapid detection of various metabolites with electrochemistry-driven regulation. Importantly, the presented SERS sensor showed considerable practicability for the detection of metabolites in real serum samples. Accordingly, the SERS sensor is a new detection platform for H₂O₂-involved metabolites detection in biological fluids, which may aid the early diagnosis of metabolism-related diseases.