TY - JOUR
T1 - Electric field directed layer-by-layer assembly of horseradish peroxidase nanotubes via anodic aluminum oxide template
AU - Wu, Fanghua
AU - Hu, Zhichao
AU - Wang, Liwei
AU - Xu, Jingjing
AU - Xian, Yuezhong
AU - Tian, Yuan
AU - Jin, Litong
PY - 2008/4
Y1 - 2008/4
N2 - A novel method based on electric field directed layer-by-layer assembly (EFDLA) and template synthesis is successfully developed to fabricate horseradish peroxidase (HRP) nanotubes. It provides a rapid and general strategy for functional protein nanoarrays. The alternative deposition of poly(diallyldimethylammonium chloride) (PDDA) and HRP are characterized by SEM, TEM, UV-vis and electrochemical impedance spectroscopy (EIS). Moreover, the UV-vis spectrometry and electrochemistry of HRP indicate that immobilization of PDDA/HRP via EFDLA coupled with template synthesis could retain the conformations and bioactivities of HRP.
AB - A novel method based on electric field directed layer-by-layer assembly (EFDLA) and template synthesis is successfully developed to fabricate horseradish peroxidase (HRP) nanotubes. It provides a rapid and general strategy for functional protein nanoarrays. The alternative deposition of poly(diallyldimethylammonium chloride) (PDDA) and HRP are characterized by SEM, TEM, UV-vis and electrochemical impedance spectroscopy (EIS). Moreover, the UV-vis spectrometry and electrochemistry of HRP indicate that immobilization of PDDA/HRP via EFDLA coupled with template synthesis could retain the conformations and bioactivities of HRP.
KW - Anodic aluminum oxide
KW - Direct electrochemistry
KW - Horseradish peroxidase
KW - Layer-by-layer assembly
KW - Nanotubes
UR - https://www.scopus.com/pages/publications/40849097414
U2 - 10.1016/j.elecom.2008.01.020
DO - 10.1016/j.elecom.2008.01.020
M3 - 文章
AN - SCOPUS:40849097414
SN - 1388-2481
VL - 10
SP - 630
EP - 634
JO - Electrochemistry Communications
JF - Electrochemistry Communications
IS - 4
ER -