TY - JOUR
T1 - Dynamic modulation of DNA hybridization using allosteric DNA tetrahedral nanostructures
AU - Song, Ping
AU - Li, Min
AU - Shen, Juwen
AU - Pei, Hao
AU - Chao, Jie
AU - Su, Shao
AU - Aldalbahi, Ali
AU - Wang, Lihua
AU - Shi, Jiye
AU - Song, Shiping
AU - Wang, Lianhui
AU - Fan, Chunhai
AU - Zuo, Xiaolei
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/8/16
Y1 - 2016/8/16
N2 - The fixed dynamic range of traditional biosensors limits their utility in several real applications. For example, viral load monitoring requires the dynamic range spans several orders of magnitude; whereas, monitoring of drugs requires extremely narrow dynamic range. To overcome this limitation, here, we devised tunable biosensing interface using allosteric DNA tetrahedral bioprobes to tune the dynamic range of DNA biosensors. Our strategy takes the advantage of the readily and flexible structure design and predictable geometric reconfiguration of DNA nanotechnology. We reconfigured the DNA tetrahedral bioprobes by inserting the effector sequence into the DNA tetrahedron, through which, the binding affinity of DNA tetrahedral bioprobes can be tuned. As a result, the detection limit of DNA biosensors can be programmably regulated. The dynamic range of DNA biosensors can be tuned (narrowed or extended) for up to 100-fold. Using the regulation of binding affinity, we realized the capture and release of biomolecules by tuning the binding behavior of DNA tetrahedral bioprobes.
AB - The fixed dynamic range of traditional biosensors limits their utility in several real applications. For example, viral load monitoring requires the dynamic range spans several orders of magnitude; whereas, monitoring of drugs requires extremely narrow dynamic range. To overcome this limitation, here, we devised tunable biosensing interface using allosteric DNA tetrahedral bioprobes to tune the dynamic range of DNA biosensors. Our strategy takes the advantage of the readily and flexible structure design and predictable geometric reconfiguration of DNA nanotechnology. We reconfigured the DNA tetrahedral bioprobes by inserting the effector sequence into the DNA tetrahedron, through which, the binding affinity of DNA tetrahedral bioprobes can be tuned. As a result, the detection limit of DNA biosensors can be programmably regulated. The dynamic range of DNA biosensors can be tuned (narrowed or extended) for up to 100-fold. Using the regulation of binding affinity, we realized the capture and release of biomolecules by tuning the binding behavior of DNA tetrahedral bioprobes.
UR - https://www.scopus.com/pages/publications/84983268832
U2 - 10.1021/acs.analchem.6b01373
DO - 10.1021/acs.analchem.6b01373
M3 - 文章
C2 - 27435955
AN - SCOPUS:84983268832
SN - 0003-2700
VL - 88
SP - 8043
EP - 8049
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 16
ER -