TY - JOUR
T1 - Dual-substrate-based full-color fluorescent probe TPE-4PA@Tb/Eu-GMP-acac ICPs for alkaline phosphatase detection and its on-site application for algal bloom alert
AU - Wu, Yuanyue
AU - Yu, Chunqian
AU - Min, Yiwen
AU - Chen, Changshun
AU - Zhou, Ke
AU - Zhai, Wanying
AU - Zhou, Tianshu
AU - Deng, Jingjing
N1 - Publisher Copyright:
© 2026 Elsevier B.V.
PY - 2026/12/1
Y1 - 2026/12/1
N2 - The development of rapid, on-site analytical methods and test strips for alkaline phosphatase activity (APA) is crucial for addressing drinking water safety crises, as it enables eutrophication assessment and algal bloom warnings under phosphorus-limited conditions. In this study, we incorporate aggregation-induced emission luminogen (AIEgen) guest TPE-4PA into bimetallic lanthanide-based infinite coordination polymers (Tb/Eu-GMP ICPs). TPE-4PA exhibits unprecedented binding preference for Tb3+ over Eu3+, triggering coordination-induced emission (CIE) at 450 nm and modulating the antenna effect (AE) and the tandem energy transfer (TER) from Tb3+ to Eu3+. Subsequently, acetylacetone (acac) was further introduced to yield TPE-4PA@Tb/Eu-GMP-acac ICPs with an appropriate full-color fluoresce response capability. The addition of alkaline phosphatase (ALP) hydrolyzes GMP and TPE-4PA dual substrates, resulting in reduced characteristic emission of the bimetallic Ln-ICP host at 493, 548, 593, 619 and 703 nm, increased monomer emission of TPE-4PA at 392 nm, as well as the solvent-driven AIE of the enzymatic product TPE-4OH at 430 nm, which constitutes a novel ratiometric ALP sensing mechanism with pale-purple to blue fluorescence color shifts. Featuring high sensitivity, doubly assured selectivity, and operational simplicity, this “all-in-one” probe enables real-time APA monitoring via dual-substrate kinetics and is suitable for on-site ALP test strip development. Moreover, the unique color transition point of the dual-substrate-based TPE-4PA@Tb/Eu-GMP-acac ICPs probe can be designed to signal an APA surge prior to algal bloom onset, which holds significant potential for the development of microalgae pollution early-warning systems, thereby safeguarding drinking water safety.
AB - The development of rapid, on-site analytical methods and test strips for alkaline phosphatase activity (APA) is crucial for addressing drinking water safety crises, as it enables eutrophication assessment and algal bloom warnings under phosphorus-limited conditions. In this study, we incorporate aggregation-induced emission luminogen (AIEgen) guest TPE-4PA into bimetallic lanthanide-based infinite coordination polymers (Tb/Eu-GMP ICPs). TPE-4PA exhibits unprecedented binding preference for Tb3+ over Eu3+, triggering coordination-induced emission (CIE) at 450 nm and modulating the antenna effect (AE) and the tandem energy transfer (TER) from Tb3+ to Eu3+. Subsequently, acetylacetone (acac) was further introduced to yield TPE-4PA@Tb/Eu-GMP-acac ICPs with an appropriate full-color fluoresce response capability. The addition of alkaline phosphatase (ALP) hydrolyzes GMP and TPE-4PA dual substrates, resulting in reduced characteristic emission of the bimetallic Ln-ICP host at 493, 548, 593, 619 and 703 nm, increased monomer emission of TPE-4PA at 392 nm, as well as the solvent-driven AIE of the enzymatic product TPE-4OH at 430 nm, which constitutes a novel ratiometric ALP sensing mechanism with pale-purple to blue fluorescence color shifts. Featuring high sensitivity, doubly assured selectivity, and operational simplicity, this “all-in-one” probe enables real-time APA monitoring via dual-substrate kinetics and is suitable for on-site ALP test strip development. Moreover, the unique color transition point of the dual-substrate-based TPE-4PA@Tb/Eu-GMP-acac ICPs probe can be designed to signal an APA surge prior to algal bloom onset, which holds significant potential for the development of microalgae pollution early-warning systems, thereby safeguarding drinking water safety.
KW - APA surge in algal blooms
KW - Ligand and AIEgen guest dual-substrates
KW - Ratiometric APA sensing and monitoring
KW - Smartphone-readable color transition point
KW - TPE-4PA@Tb/Eu-GMP-Acac ICPs
KW - Visual alert systems
UR - https://www.scopus.com/pages/publications/105042556114
U2 - 10.1016/j.talanta.2026.130159
DO - 10.1016/j.talanta.2026.130159
M3 - 文章
AN - SCOPUS:105042556114
SN - 0039-9140
VL - 310
JO - Talanta
JF - Talanta
M1 - 130159
ER -