TY - JOUR
T1 - Dual emission fluorescent silver nanoclusters for sensitive detection of the biological coenzyme NAD+/NADH
AU - Yuan, Yufeng
AU - Huang, Kehan
AU - Chang, Mengfang
AU - Qin, Cuifang
AU - Zhang, Sanjun
AU - Pan, Haifeng
AU - Chen, Yan
AU - Xu, Jianhua
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Fluorescent silver nanoclusters (Ag NCs) displaying dual-excitation and dual-emission properties have been developed for the specific detection of NAD+ (nicotinamide adenine dinucleotide, oxidized form). With the increase of NAD+ concentrations, the longer wavelength emission (with the peak at 550 nm) was gradually quenched due to the strong interactions between the NAD+ and Ag NCs, whereas the shorter wavelength emission (peaking at 395 nm) was linearly enhanced. More important, the dual-emission intensity ratio (I395/I550), fitting by a single-exponential decay function, can efficiently detect various NAD+ levels from 100 to 4000 μM, as well as label NAD+/NADH (reduced form of NAD) ratios in the range of 1-50.
AB - Fluorescent silver nanoclusters (Ag NCs) displaying dual-excitation and dual-emission properties have been developed for the specific detection of NAD+ (nicotinamide adenine dinucleotide, oxidized form). With the increase of NAD+ concentrations, the longer wavelength emission (with the peak at 550 nm) was gradually quenched due to the strong interactions between the NAD+ and Ag NCs, whereas the shorter wavelength emission (peaking at 395 nm) was linearly enhanced. More important, the dual-emission intensity ratio (I395/I550), fitting by a single-exponential decay function, can efficiently detect various NAD+ levels from 100 to 4000 μM, as well as label NAD+/NADH (reduced form of NAD) ratios in the range of 1-50.
KW - Biological coenzyme NAD
KW - Dual-emission fluorescent silver nanoclusters
KW - Dual-emission intensity ratio
KW - NAD/NADH ratios
UR - https://www.scopus.com/pages/publications/84948442632
U2 - 10.1016/j.ab.2015.09.021
DO - 10.1016/j.ab.2015.09.021
M3 - 文章
C2 - 26452612
AN - SCOPUS:84948442632
SN - 0003-2697
VL - 494
SP - 46
EP - 48
JO - Analytical Biochemistry
JF - Analytical Biochemistry
ER -