DNA hybridization detection with blue luminescent quantum dots and dye-labeled single-stranded DNA

Hui Peng; Lijuan Zhang; Tanja H.M. Kjällman; Christian Soeller; Jadranka Travas-Sejdic

doi:10.1021/ja0685452

DNA hybridization detection with blue luminescent quantum dots and dye-labeled single-stranded DNA

Hui Peng
, Lijuan Zhang
, Tanja H.M. Kjällman
, Christian Soeller
, Jadranka Travas-Sejdic^*

^*此作品的通讯作者

The University of Auckland

科研成果: 期刊稿件 › 文章 › 同行评审

274 引用（Scopus）

摘要

A simple DNA sensing platform was developed based on the fluorescence resonance energy transfer (FRET) between blue-luminescent CdTe QDs and dye-labeled ssDNA. A cationic polymer acts as a "bridge" to bring the donor to acceptor together and achieves the high FRET efficiency. The hybridization event was recognized by the different FRET efficiencies due to different interaction abilities of single-stranded and double-stranded DNA with the polymer. This platform provides a homogeneous DNA assay that takes advantage of the fluorescence detection method, but with minimal DNA modification.

源语言	英语
页（从-至）	3048-3049
页数	2
期刊	Journal of the American Chemical Society
卷	129
期	11
DOI	https://doi.org/10.1021/ja0685452
出版状态	已出版 - 21 3月 2007
已对外发布	是

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AU - Peng, Hui

AU - Zhang, Lijuan

AU - Kjällman, Tanja H.M.

AU - Soeller, Christian

AU - Travas-Sejdic, Jadranka

PY - 2007/3/21

Y1 - 2007/3/21

N2 - A simple DNA sensing platform was developed based on the fluorescence resonance energy transfer (FRET) between blue-luminescent CdTe QDs and dye-labeled ssDNA. A cationic polymer acts as a "bridge" to bring the donor to acceptor together and achieves the high FRET efficiency. The hybridization event was recognized by the different FRET efficiencies due to different interaction abilities of single-stranded and double-stranded DNA with the polymer. This platform provides a homogeneous DNA assay that takes advantage of the fluorescence detection method, but with minimal DNA modification.

AB - A simple DNA sensing platform was developed based on the fluorescence resonance energy transfer (FRET) between blue-luminescent CdTe QDs and dye-labeled ssDNA. A cationic polymer acts as a "bridge" to bring the donor to acceptor together and achieves the high FRET efficiency. The hybridization event was recognized by the different FRET efficiencies due to different interaction abilities of single-stranded and double-stranded DNA with the polymer. This platform provides a homogeneous DNA assay that takes advantage of the fluorescence detection method, but with minimal DNA modification.

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ER -

DNA hybridization detection with blue luminescent quantum dots and dye-labeled single-stranded DNA

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