DNA-hosted Hoechst dyes: Application for label-free fluorescent monitoring of endonuclease activity and inhibition

Xiao Qin Jiang; Su Miao Guo; Min Zhang; Ming Zhou; Bang Ce Ye

doi:10.1039/c4an01373e

DNA-hosted Hoechst dyes: Application for label-free fluorescent monitoring of endonuclease activity and inhibition

Xiao Qin Jiang
, Su Miao Guo
, Min Zhang^*
, Ming Zhou
, Bang Ce Ye

^*此作品的通讯作者

化学与分子工程学院

East China University of Science and Technology
University of Washington

科研成果: 期刊稿件 › 文章 › 同行评审

14 引用（Scopus）

摘要

A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.

源语言	英语
页（从-至）	5682-5685
页数	4
期刊	Analyst
卷	139
期	22
DOI	https://doi.org/10.1039/c4an01373e
出版状态	已出版 - 15 10月 2014

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@article{4626f2aadaf141ce887c664500409d0f,

title = "DNA-hosted Hoechst dyes: Application for label-free fluorescent monitoring of endonuclease activity and inhibition",

abstract = "A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.",

author = "Jiang, \{Xiao Qin\} and Guo, \{Su Miao\} and Min Zhang and Ming Zhou and Ye, \{Bang Ce\}",

year = "2014",

month = oct,

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doi = "10.1039/c4an01373e",

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TY - JOUR

T1 - DNA-hosted Hoechst dyes

T2 - Application for label-free fluorescent monitoring of endonuclease activity and inhibition

AU - Jiang, Xiao Qin

AU - Guo, Su Miao

AU - Zhang, Min

AU - Zhou, Ming

AU - Ye, Bang Ce

PY - 2014/10/15

Y1 - 2014/10/15

N2 - A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.

AB - A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.

UR - https://www.scopus.com/pages/publications/84907989518

U2 - 10.1039/c4an01373e

DO - 10.1039/c4an01373e

M3 - 文章

C2 - 25272021

AN - SCOPUS:84907989518

SN - 0003-2654

VL - 139

SP - 5682

EP - 5685

JO - Analyst

JF - Analyst

IS - 22

ER -

DNA-hosted Hoechst dyes: Application for label-free fluorescent monitoring of endonuclease activity and inhibition

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