Dip-and-read method for label-free renewable sensing enhanced using complex DNA structures

Min Zhang; Xiao Qin Jiang; Huynh Nhu Le; Ping Wang; Bang Ce Ye

doi:10.1021/am302321m

Dip-and-read method for label-free renewable sensing enhanced using complex DNA structures

Min Zhang
, Xiao Qin Jiang
, Huynh Nhu Le
, Ping Wang^*
, Bang Ce Ye

^*此作品的通讯作者

East China University of Science and Technology

科研成果: 期刊稿件 › 文章 › 同行评审

13 引用（Scopus）

摘要

A label-free assay is reported in this work for the detection of DNA with enhanced sensitivity using complex DNA structures (DNA tetrahedrons) based on the biolayer interferometry. The DNA tetrahedrons help to amplify the optical signals of the biolayer interferometry, thus improving the detection limit of DNA by about 100-fold. We further demonstrated that this method could be expanded to ATP detection by taking advantage of the target-dependent adaptability of aptamers. It appears to us that this new label-free assay promises new opportunities for developing novel biolayer interferometry assays.

源语言	英语
页（从-至）	473-478
页数	6
期刊	ACS Applied Materials and Interfaces
卷	5
期	3
DOI	https://doi.org/10.1021/am302321m
出版状态	已出版 - 13 2月 2013
已对外发布	是

联合国可持续发展目标

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可持续发展目标 7 经济适用的清洁能源

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title = "Dip-and-read method for label-free renewable sensing enhanced using complex DNA structures",

abstract = "A label-free assay is reported in this work for the detection of DNA with enhanced sensitivity using complex DNA structures (DNA tetrahedrons) based on the biolayer interferometry. The DNA tetrahedrons help to amplify the optical signals of the biolayer interferometry, thus improving the detection limit of DNA by about 100-fold. We further demonstrated that this method could be expanded to ATP detection by taking advantage of the target-dependent adaptability of aptamers. It appears to us that this new label-free assay promises new opportunities for developing novel biolayer interferometry assays.",

keywords = "DNA detection, DNA tetrahedron, aptamer, label-free, signal amplification, dip-and-read",

author = "Min Zhang and Jiang, \{Xiao Qin\} and Le, \{Huynh Nhu\} and Ping Wang and Ye, \{Bang Ce\}",

year = "2013",

month = feb,

day = "13",

doi = "10.1021/am302321m",

language = "英语",

volume = "5",

pages = "473--478",

journal = "ACS Applied Materials and Interfaces",

issn = "1944-8244",

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TY - JOUR

T1 - Dip-and-read method for label-free renewable sensing enhanced using complex DNA structures

AU - Zhang, Min

AU - Jiang, Xiao Qin

AU - Le, Huynh Nhu

AU - Wang, Ping

AU - Ye, Bang Ce

PY - 2013/2/13

Y1 - 2013/2/13

N2 - A label-free assay is reported in this work for the detection of DNA with enhanced sensitivity using complex DNA structures (DNA tetrahedrons) based on the biolayer interferometry. The DNA tetrahedrons help to amplify the optical signals of the biolayer interferometry, thus improving the detection limit of DNA by about 100-fold. We further demonstrated that this method could be expanded to ATP detection by taking advantage of the target-dependent adaptability of aptamers. It appears to us that this new label-free assay promises new opportunities for developing novel biolayer interferometry assays.

AB - A label-free assay is reported in this work for the detection of DNA with enhanced sensitivity using complex DNA structures (DNA tetrahedrons) based on the biolayer interferometry. The DNA tetrahedrons help to amplify the optical signals of the biolayer interferometry, thus improving the detection limit of DNA by about 100-fold. We further demonstrated that this method could be expanded to ATP detection by taking advantage of the target-dependent adaptability of aptamers. It appears to us that this new label-free assay promises new opportunities for developing novel biolayer interferometry assays.

KW - DNA detection

KW - DNA tetrahedron

KW - aptamer

KW - label-free

KW - signal amplification, dip-and-read

UR - https://www.scopus.com/pages/publications/84873637187

U2 - 10.1021/am302321m

DO - 10.1021/am302321m

M3 - 文章

C2 - 23298262

AN - SCOPUS:84873637187

SN - 1944-8244

VL - 5

SP - 473

EP - 478

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 3

ER -

Dip-and-read method for label-free renewable sensing enhanced using complex DNA structures

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