TY - JOUR
T1 - Dietary vitamin A affects growth performance, immunity, antioxidant capacity, and lipid metabolism of juvenile Chinese mitten crab Eriocheir sinensis
AU - Huang, Qincheng
AU - Wang, Xiaodan
AU - Bu, Xianyong
AU - Song, Ying
AU - You, Junfeng
AU - Zhang, Cong
AU - Qin, Chuanjie
AU - Qin, Jianguang
AU - Chen, Liqiao
N1 - Publisher Copyright:
© 2021
PY - 2022/2/15
Y1 - 2022/2/15
N2 - The effects of dietary vitamin A level on the growth performance, immunity, oxidation resistance and lipid metabolism of juvenile Chinese mitten crab Eriocheir sinensis (0.84 ± 0.00 g) were determined in the present study. Six group of crabs (four replicate tanks per group with 40 crabs per tank) were respectively fed with 0 (control), 2322, 4511, 8478, 14,333 and 30,444 IU/kg dietary vitamin A for 8 weeks. The weight gain (WG) and specific growth rate (SGR) of juvenile crabs significantly increased in dietary vitamin A supplement groups compared with the control. Dietary vitamin A supplementation increased immunity and antioxidant capacity, hepatopancreas health and alleviated lipid peroxidation in the hepatopancreas of crabs. Moreover, total lipid content in the whole body and hepatopancreas, and triglyceride (TG) content in the hepatopancreas and serum of crabs showed a decreasing tendency with increasing inclusion of vitamin A. The hepatosomatic index (HSI) was lowest in crabs fed 30,444 IU/kg dietary vitamin A. Interestingly, the transcriptome sequencing data showed that the expression of genes related to immunity, antioxidant capacity, lipolysis and β-oxidation was up-regulated. In contrast, the expression of genes related to lipid synthesis was down-regulated in crabs fed 30,444 IU/kg dietary vitamin A compared with the control. This study indicates that dietary vitamin A requirement for E. sinensis is 5340 for WG and 5540 IU/kg for SGR based on the broken-line model.
AB - The effects of dietary vitamin A level on the growth performance, immunity, oxidation resistance and lipid metabolism of juvenile Chinese mitten crab Eriocheir sinensis (0.84 ± 0.00 g) were determined in the present study. Six group of crabs (four replicate tanks per group with 40 crabs per tank) were respectively fed with 0 (control), 2322, 4511, 8478, 14,333 and 30,444 IU/kg dietary vitamin A for 8 weeks. The weight gain (WG) and specific growth rate (SGR) of juvenile crabs significantly increased in dietary vitamin A supplement groups compared with the control. Dietary vitamin A supplementation increased immunity and antioxidant capacity, hepatopancreas health and alleviated lipid peroxidation in the hepatopancreas of crabs. Moreover, total lipid content in the whole body and hepatopancreas, and triglyceride (TG) content in the hepatopancreas and serum of crabs showed a decreasing tendency with increasing inclusion of vitamin A. The hepatosomatic index (HSI) was lowest in crabs fed 30,444 IU/kg dietary vitamin A. Interestingly, the transcriptome sequencing data showed that the expression of genes related to immunity, antioxidant capacity, lipolysis and β-oxidation was up-regulated. In contrast, the expression of genes related to lipid synthesis was down-regulated in crabs fed 30,444 IU/kg dietary vitamin A compared with the control. This study indicates that dietary vitamin A requirement for E. sinensis is 5340 for WG and 5540 IU/kg for SGR based on the broken-line model.
KW - Eriocheir sinensis
KW - Growth performance
KW - Immunity and antioxidant capacity
KW - Lipid metabolism
KW - Vitamin A
UR - https://www.scopus.com/pages/publications/85116534534
U2 - 10.1016/j.aquaculture.2021.737556
DO - 10.1016/j.aquaculture.2021.737556
M3 - 文章
AN - SCOPUS:85116534534
SN - 0044-8486
VL - 548
JO - Aquaculture
JF - Aquaculture
M1 - 737556
ER -