Development of polymorphic microsatellite markers for Ginkgo biloba L. by database mining

Ginkgo biloba L. is a famous living fossil plant endemic to China. We report twelve polymorphic simple sequence repeat (SSRs) markers of G. biloba by mining expressed sequence tags (ESTs). One locus was significantly deviated from Hardy-Weinberg equilibrium. The number of alleles per locus ranged from 2 to 5. The PIC values were from 0.058 to 0.776. Observed and expected heterozygosity ranged from 0.050 to 0.776, and 0.055 to 0.799, respectively. These markers will be available for studies of population genetics, reproductive ecology and conservation genetics for G. biloba.