TY - JOUR
T1 - Determination of isatin and monoamine neurotransmitters in rat brain with liquid chromatography using palladium hexacyanoferrate modified electrode
AU - Xu, Haihong
AU - Wang, Dan
AU - Zhang, Wen
AU - Zhu, Wei
AU - Yamamoto, Katsunobu
AU - Jin, Litong
PY - 2006/9/8
Y1 - 2006/9/8
N2 - The fabrication and application of a novel electrochemical detector (ED) with palladium hexacyanoferrate (PdHCF) chemically modified electrode (CME) for liquid chromatography (LC) were described. The electrochemical behaviors of isatin, monoamine neurotransmitters and their metabolites at this CME were investigated by cyclic voltammetry. It was found that the CME exhibited efficiently electrocatalytic of isatin and showed high sensitivity and stability for determination of monoamine neurotransmitters. The linear ranges were over three orders of magnitude and the detection limits were 2.5 × 10-8 mol L-1 for isatin, 2.5 × 10-10 mol L-1 for norepinephrine (NE), 2.5 × 10-10 mol L-1 for 5-hydroxyindoleacetic acid (5-HIAA), 5.0 × 10-10 mol L-1 for dopamine (DA), 1.0 × 10-9 mol L-1 for 3,4-dihydroxyphenylacetic acid (DOPAC), 1.2 × 10-10 mol L-1 for 5-hydroxytryptamine (5-HT) and 2.5 × 10-9 mol L-1 for homovanillic acid (HVA). Combined with microdialysis, the method was successfully applied to study the effect of isatin on the levels of monoamine neurotransmitters in experimental Parkinsonian rats. The results showed that isatin could significantly increase striatal monoamine neurotransmitters release to the basal level.
AB - The fabrication and application of a novel electrochemical detector (ED) with palladium hexacyanoferrate (PdHCF) chemically modified electrode (CME) for liquid chromatography (LC) were described. The electrochemical behaviors of isatin, monoamine neurotransmitters and their metabolites at this CME were investigated by cyclic voltammetry. It was found that the CME exhibited efficiently electrocatalytic of isatin and showed high sensitivity and stability for determination of monoamine neurotransmitters. The linear ranges were over three orders of magnitude and the detection limits were 2.5 × 10-8 mol L-1 for isatin, 2.5 × 10-10 mol L-1 for norepinephrine (NE), 2.5 × 10-10 mol L-1 for 5-hydroxyindoleacetic acid (5-HIAA), 5.0 × 10-10 mol L-1 for dopamine (DA), 1.0 × 10-9 mol L-1 for 3,4-dihydroxyphenylacetic acid (DOPAC), 1.2 × 10-10 mol L-1 for 5-hydroxytryptamine (5-HT) and 2.5 × 10-9 mol L-1 for homovanillic acid (HVA). Combined with microdialysis, the method was successfully applied to study the effect of isatin on the levels of monoamine neurotransmitters in experimental Parkinsonian rats. The results showed that isatin could significantly increase striatal monoamine neurotransmitters release to the basal level.
KW - High performance liquid chromatography-electrochemical detection
KW - Isatin
KW - Monoamine neurotransmitters
KW - Palladium hexacyanoferrate
KW - Parkinson's disease
UR - https://www.scopus.com/pages/publications/33748156632
U2 - 10.1016/j.aca.2006.06.042
DO - 10.1016/j.aca.2006.06.042
M3 - 文章
AN - SCOPUS:33748156632
SN - 0003-2670
VL - 577
SP - 207
EP - 213
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 2
ER -