CO₂ bubbling-based 'Nanobomb' system for targetedly suppressing Panc-1 pancreatic tumor via low intensity ultrasound-activated inertial cavitation

Noninvasive and targeted physical treatment is still desirable especially for those cancerous patients. Herein, we develop a new physical treatment protocol by employing CO₂ bubbling-based 'nanobomb' system consisting of low-intensity ultrasound (1.0 W/cm²) and a well-constructed pH/temperature dual-responsive CO₂ release system. Depending on the temperature elevation caused by exogenous low-intensity therapeutic ultrasound irradiation and the low pH caused by the endogenous acidic-environment around/within tumor, dual-responsive CO₂ release system can quickly release CO₂ bubbles, and afterwards, the generated CO₂ bubbles waves will timely explode before dissolution due to triggering by therapeutic ultrasound waves. Related bio-effects (e.g., cavitation, mechanical, shock waves, etc) caused by CO₂ bubbles' explosion effectively induce instant necrosis of panc-1 cells and blood vessel destruction within panc-1 tumor, and consequently inhibit the growth of panc-1 solid tumor, simultaneously minimizing the side effects to normal organs. This new physiotherapy employing CO₂ bubbling-based 'nanobomb' system promises significant potentials in targetedly suppressing tumors, especially for those highly deadly cancers.