Construction and Identification of DNA vaccine containing chimeric gene gag-gp120 of HIV-1

Wen Zheng Jiang; Ning Yi Jin; Wen Yu Han

Construction and Identification of DNA vaccine containing chimeric gene gag-gp120 of HIV-1

Wen Zheng Jiang
, Ning Yi Jin
, Wen Yu Han

Quartermaster University of PLA

科研成果: 期刊稿件 › 文章 › 同行评审

摘要

AIM: To construct DNA vaccine expression plasmid containing the chimeric gene gag-gp120 of HIV-1.

METHODS: The recombinant eukaryotic expression vector pVAXGE was constructed via inserting the chimeric gene gag-gp120 into the vector pVAX1. Hela cells had been transfected by recombinant plasmid via liposome. After 72 h, the transfected cells was detected by RT-PCR and analyzed by Dot-ELISA.

RESULTS: The transcript products of target gene could be amplified from the cells transfected by recombinant plasmid. Dot-ELISA detection showed that the target gene was expressed in Hela cells.

CONCLUSION: The DNA vaccine plasmid expressing chimeric gene gag-gp120 was successfully constructed, which lays the foundation for preparing DNA vaccine against HIV-1.

源语言	英语
页（从-至）	341-342
页数	2
期刊	Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
卷	19
期	4
出版状态	已出版 - 1 7月 2003
已对外发布	是

联合国可持续发展目标

此成果有助于实现下列可持续发展目标：

可持续发展目标 3 良好健康与福祉

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title = "Construction and Identification of DNA vaccine containing chimeric gene gag-gp120 of HIV-1",

abstract = "AIM: To construct DNA vaccine expression plasmid containing the chimeric gene gag-gp120 of HIV-1.METHODS: The recombinant eukaryotic expression vector pVAXGE was constructed via inserting the chimeric gene gag-gp120 into the vector pVAX1. Hela cells had been transfected by recombinant plasmid via liposome. After 72 h, the transfected cells was detected by RT-PCR and analyzed by Dot-ELISA.RESULTS: The transcript products of target gene could be amplified from the cells transfected by recombinant plasmid. Dot-ELISA detection showed that the target gene was expressed in Hela cells.CONCLUSION: The DNA vaccine plasmid expressing chimeric gene gag-gp120 was successfully constructed, which lays the foundation for preparing DNA vaccine against HIV-1.",

author = "Jiang, \{Wen Zheng\} and Jin, \{Ning Yi\} and Han, \{Wen Yu\}",

year = "2003",

month = jul,

day = "1",

language = "英语",

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pages = "341--342",

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T1 - Construction and Identification of DNA vaccine containing chimeric gene gag-gp120 of HIV-1

AU - Jiang, Wen Zheng

AU - Jin, Ning Yi

AU - Han, Wen Yu

PY - 2003/7/1

Y1 - 2003/7/1

N2 - AIM: To construct DNA vaccine expression plasmid containing the chimeric gene gag-gp120 of HIV-1.METHODS: The recombinant eukaryotic expression vector pVAXGE was constructed via inserting the chimeric gene gag-gp120 into the vector pVAX1. Hela cells had been transfected by recombinant plasmid via liposome. After 72 h, the transfected cells was detected by RT-PCR and analyzed by Dot-ELISA.RESULTS: The transcript products of target gene could be amplified from the cells transfected by recombinant plasmid. Dot-ELISA detection showed that the target gene was expressed in Hela cells.CONCLUSION: The DNA vaccine plasmid expressing chimeric gene gag-gp120 was successfully constructed, which lays the foundation for preparing DNA vaccine against HIV-1.

AB - AIM: To construct DNA vaccine expression plasmid containing the chimeric gene gag-gp120 of HIV-1.METHODS: The recombinant eukaryotic expression vector pVAXGE was constructed via inserting the chimeric gene gag-gp120 into the vector pVAX1. Hela cells had been transfected by recombinant plasmid via liposome. After 72 h, the transfected cells was detected by RT-PCR and analyzed by Dot-ELISA.RESULTS: The transcript products of target gene could be amplified from the cells transfected by recombinant plasmid. Dot-ELISA detection showed that the target gene was expressed in Hela cells.CONCLUSION: The DNA vaccine plasmid expressing chimeric gene gag-gp120 was successfully constructed, which lays the foundation for preparing DNA vaccine against HIV-1.

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JO - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology

JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology

IS - 4

ER -

Construction and Identification of DNA vaccine containing chimeric gene gag-gp120 of HIV-1

摘要

联合国可持续发展目标

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指纹

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