Comparative study of two methods on determination of hydrogen peroxide

A hydrogen peroxide biosensor has been made by horseradish peroxidase/polyvinyl butyral/multiwall carbon nanotube immobilizing on the glassy carbon electrode surface. Using hydroquinone as mediator, the electrocatalytic properties of this sensor toward hydrogen peroxide are observed by i-t curves, which are compared with ultraviolet spectrophotometry. The biosensor exhibits easy-making, simple operation, quick inspection, wider linear range and lower detection limit ability. In contrast, ultraviolet spectrophotometry has advantages in reproducibility, stability and noise immunity.