Cloning of a cDNA encoding an Acanthamoeba castellanii PDI-like protein

Jie Min Wong; Erik Bateman

doi:10.1016/0378-1119(94)90880-X

Cloning of a cDNA encoding an Acanthamoeba castellanii PDI-like protein

Jie Min Wong
, Erik Bateman^*

^*此作品的通讯作者

University of Vermont

科研成果: 期刊稿件 › 文章 › 同行评审

4 引用（Scopus）

摘要

We have cloned, from Acanthamoeba castellanii, the cDNA encoding a new member of the protein disulfide isomerase (PDI)-like protein family. The new PDI-like protein contains two highly conserved thioredeoxin-like domains, each about 100 amino acids in length. However, the A. castellanii PDI-like protein differs from other members in many aspects, including the overall organization and isoelectric point. Southern and Northern analyses demonstrate that the PDI-like protein is encoded by a single-copy gene which is transcribed to generate a 1500-nucleotide mRNA.

源语言	英语
页（从-至）	175-179
页数	5
期刊	Gene
卷	150
期	1
DOI	https://doi.org/10.1016/0378-1119(94)90880-X
出版状态	已出版 - 2 12月 1994
已对外发布	是

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title = "Cloning of a cDNA encoding an Acanthamoeba castellanii PDI-like protein",

abstract = "We have cloned, from Acanthamoeba castellanii, the cDNA encoding a new member of the protein disulfide isomerase (PDI)-like protein family. The new PDI-like protein contains two highly conserved thioredeoxin-like domains, each about 100 amino acids in length. However, the A. castellanii PDI-like protein differs from other members in many aspects, including the overall organization and isoelectric point. Southern and Northern analyses demonstrate that the PDI-like protein is encoded by a single-copy gene which is transcribed to generate a 1500-nucleotide mRNA.",

keywords = "Recombinant DNA, gene cloning, protein disulfide isomerase",

author = "Wong, \{Jie Min\} and Erik Bateman",

year = "1994",

month = dec,

day = "2",

doi = "10.1016/0378-1119(94)90880-X",

language = "英语",

volume = "150",

pages = "175--179",

journal = "Gene",

issn = "0378-1119",

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TY - JOUR

T1 - Cloning of a cDNA encoding an Acanthamoeba castellanii PDI-like protein

AU - Wong, Jie Min

AU - Bateman, Erik

PY - 1994/12/2

Y1 - 1994/12/2

N2 - We have cloned, from Acanthamoeba castellanii, the cDNA encoding a new member of the protein disulfide isomerase (PDI)-like protein family. The new PDI-like protein contains two highly conserved thioredeoxin-like domains, each about 100 amino acids in length. However, the A. castellanii PDI-like protein differs from other members in many aspects, including the overall organization and isoelectric point. Southern and Northern analyses demonstrate that the PDI-like protein is encoded by a single-copy gene which is transcribed to generate a 1500-nucleotide mRNA.

AB - We have cloned, from Acanthamoeba castellanii, the cDNA encoding a new member of the protein disulfide isomerase (PDI)-like protein family. The new PDI-like protein contains two highly conserved thioredeoxin-like domains, each about 100 amino acids in length. However, the A. castellanii PDI-like protein differs from other members in many aspects, including the overall organization and isoelectric point. Southern and Northern analyses demonstrate that the PDI-like protein is encoded by a single-copy gene which is transcribed to generate a 1500-nucleotide mRNA.

KW - Recombinant DNA

KW - gene cloning

KW - protein disulfide isomerase

UR - https://www.scopus.com/pages/publications/0027987374

U2 - 10.1016/0378-1119(94)90880-X

DO - 10.1016/0378-1119(94)90880-X

M3 - 文章

C2 - 7959048

AN - SCOPUS:0027987374

SN - 0378-1119

VL - 150

SP - 175

EP - 179

JO - Gene

JF - Gene

IS - 1

ER -

Cloning of a cDNA encoding an Acanthamoeba castellanii PDI-like protein

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