Characterization and characteristics of poly (γ-glutamic acids)-aspartic acid polymer

Poly (γ-glutamic acids) was obtained by using fermentation methods. The small molecular weight of poly (γ-glutamic acids) was prepared by acid hydrolysis. The aspartic acid as a side chain was linked to poly (γ-glutamic acids), and poly (γ-glutamic acids)-aspartic acid (GAP460) was prepared. The polymer was characterized by ¹H-NMR, X-ray and differential scanning calorimetry. Structural stability, immunogenicity, acute toxicity and principal haematological parameters of GAP460 were examined by routine methods. Results reveal GAP460 is obtained and structural of GAP460 is stable in the conditions of neutral, room temperature and 4°C, on eight weeks after first immunization, the serum antibody titer of rats is 1:20, GAP460 shows good safety and low immunogenicity in rats, acute toxicity and principal haematological parameters studies show GAP460 displays atoxic. Our results suggest that GAP460 can be used as an effective carrier for drug delivery and may have potential applications.