TY - JOUR
T1 - A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au nanocomposite for horseradish peroxidase immobilization and biosensing
AU - Xu, Jingjing
AU - Peng, Ru
AU - Ran, Qin
AU - Xian, Yuezhong
AU - Tian, Yuan
AU - Jin, Litong
PY - 2010/9/15
Y1 - 2010/9/15
N2 - A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au (PEDOT-PSS/Au) nanocomposite was prepared via one-step chemical synthesis and the matrix was characterized by UV-vis spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and transmission electron microscope (TEM). Due to the excellent aqueous compatibility and biocompatibility, the PEDOT/PSS-Au nanocomposite can be used as biomaterial for enzymes immobilization. In this system, redox enzyme, horseradish peroxidase (HRP) was integrated with PEDOT/PSS-Au nanocomposite and the direct electron transfer of HRP was observed. Moreover, we find that the HRP/PEDOT-PSS/Au modified electrode shows excellent electrocatalytic ability for H2O2 and the formal Michaelis-Menten constant (Kmapp) was 0.78 mmol/L. The response currents have good linear relation with the concentrations of H2O2 with a linear range from 2.0 × 10-7 to 3.8 × 10-4 mol/L.
AB - A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au (PEDOT-PSS/Au) nanocomposite was prepared via one-step chemical synthesis and the matrix was characterized by UV-vis spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and transmission electron microscope (TEM). Due to the excellent aqueous compatibility and biocompatibility, the PEDOT/PSS-Au nanocomposite can be used as biomaterial for enzymes immobilization. In this system, redox enzyme, horseradish peroxidase (HRP) was integrated with PEDOT/PSS-Au nanocomposite and the direct electron transfer of HRP was observed. Moreover, we find that the HRP/PEDOT-PSS/Au modified electrode shows excellent electrocatalytic ability for H2O2 and the formal Michaelis-Menten constant (Kmapp) was 0.78 mmol/L. The response currents have good linear relation with the concentrations of H2O2 with a linear range from 2.0 × 10-7 to 3.8 × 10-4 mol/L.
KW - Au nanopartiles
KW - Biosensor
KW - Direct electrochemistry
KW - Horseradish peroxidase
KW - Hydrogen peroxide
KW - PEDOT-PSS conductive polymer
UR - https://www.scopus.com/pages/publications/77956186986
U2 - 10.1016/j.talanta.2010.07.033
DO - 10.1016/j.talanta.2010.07.033
M3 - 文章
AN - SCOPUS:77956186986
SN - 0039-9140
VL - 82
SP - 1511
EP - 1515
JO - Talanta
JF - Talanta
IS - 4
ER -