A DPA-based highly selective and sensitive fluorescent probe for mercuric ions and its imaging in living cells

Junhua Jia; Luling Wu; Yu Ding; Chusen Huang; Weiping Zhu; Yufang Xu; Xuhong Qian

doi:10.1039/c6dt01258b

A DPA-based highly selective and sensitive fluorescent probe for mercuric ions and its imaging in living cells

Junhua Jia
, Luling Wu
, Yu Ding
, Chusen Huang^*
, Weiping Zhu
, Yufang Xu
, Xuhong Qian

^*此作品的通讯作者

East China University of Science and Technology
Shanghai Normal University

科研成果: 期刊稿件 › 文章 › 同行评审

16 引用（Scopus）

摘要

Based on the 2,2-dipicolylamine (DPA) receptor and naphthalimide fluorophore, three fluorescent probes, RDPA, MDPA and VDPA have been developed for the recognition of mercuric ions. Among them, VDPA, bearing a diglycol group in the imine chain, exhibits good water solubility, and high selectivity towards mercuric ions in PBS water solution. With DPA as the receptor, VDPA showed very high affinity and sensitivity towards Hg²⁺, and the binding constant and detection limit were calculated to be 2.8 × 10¹⁰ M^-1 and 5.49 nM, respectively. The MTT assay and living cell imaging experiments suggested that the probe VDPA has potential application for detecting Hg²⁺ in living cells.

源语言	英语
页（从-至）	9402-9406
页数	5
期刊	Dalton Transactions
卷	45
期	23
DOI	https://doi.org/10.1039/c6dt01258b
出版状态	已出版 - 2016
已对外发布	是

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title = "A DPA-based highly selective and sensitive fluorescent probe for mercuric ions and its imaging in living cells",

abstract = "Based on the 2,2-dipicolylamine (DPA) receptor and naphthalimide fluorophore, three fluorescent probes, RDPA, MDPA and VDPA have been developed for the recognition of mercuric ions. Among them, VDPA, bearing a diglycol group in the imine chain, exhibits good water solubility, and high selectivity towards mercuric ions in PBS water solution. With DPA as the receptor, VDPA showed very high affinity and sensitivity towards Hg2+, and the binding constant and detection limit were calculated to be 2.8 × 1010 M-1 and 5.49 nM, respectively. The MTT assay and living cell imaging experiments suggested that the probe VDPA has potential application for detecting Hg2+ in living cells.",

author = "Junhua Jia and Luling Wu and Yu Ding and Chusen Huang and Weiping Zhu and Yufang Xu and Xuhong Qian",

note = "Publisher Copyright: {\textcopyright} 2016 The Royal Society of Chemistry.",

year = "2016",

doi = "10.1039/c6dt01258b",

language = "英语",

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pages = "9402--9406",

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TY - JOUR

T1 - A DPA-based highly selective and sensitive fluorescent probe for mercuric ions and its imaging in living cells

AU - Jia, Junhua

AU - Wu, Luling

AU - Ding, Yu

AU - Huang, Chusen

AU - Zhu, Weiping

AU - Xu, Yufang

AU - Qian, Xuhong

PY - 2016

Y1 - 2016

N2 - Based on the 2,2-dipicolylamine (DPA) receptor and naphthalimide fluorophore, three fluorescent probes, RDPA, MDPA and VDPA have been developed for the recognition of mercuric ions. Among them, VDPA, bearing a diglycol group in the imine chain, exhibits good water solubility, and high selectivity towards mercuric ions in PBS water solution. With DPA as the receptor, VDPA showed very high affinity and sensitivity towards Hg2+, and the binding constant and detection limit were calculated to be 2.8 × 1010 M-1 and 5.49 nM, respectively. The MTT assay and living cell imaging experiments suggested that the probe VDPA has potential application for detecting Hg2+ in living cells.

AB - Based on the 2,2-dipicolylamine (DPA) receptor and naphthalimide fluorophore, three fluorescent probes, RDPA, MDPA and VDPA have been developed for the recognition of mercuric ions. Among them, VDPA, bearing a diglycol group in the imine chain, exhibits good water solubility, and high selectivity towards mercuric ions in PBS water solution. With DPA as the receptor, VDPA showed very high affinity and sensitivity towards Hg2+, and the binding constant and detection limit were calculated to be 2.8 × 1010 M-1 and 5.49 nM, respectively. The MTT assay and living cell imaging experiments suggested that the probe VDPA has potential application for detecting Hg2+ in living cells.

UR - https://www.scopus.com/pages/publications/84973901426

U2 - 10.1039/c6dt01258b

DO - 10.1039/c6dt01258b

M3 - 文章

C2 - 27221695

AN - SCOPUS:84973901426

SN - 1477-9226

VL - 45

SP - 9402

EP - 9406

JO - Dalton Transactions

JF - Dalton Transactions

IS - 23

ER -

A DPA-based highly selective and sensitive fluorescent probe for mercuric ions and its imaging in living cells

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