微流控技术制备ZnO纳米棒及生物荧光检测性能研究

A simple microfluidic chip was designed and fabricated, on which zinc oxide (ZnO) nanorods were synthesized by hydrothermal method. The influence of growth condition on morphology and crystal structure of ZnO nanorods were investigated by scanning electron microscope (SEM) and X-ray diffraction (XRD), respectively. Experimental results showed that densely packed ZnO nanorods could be prepared on microfluidic chip, with good crystallinity and c-axis orientation. The diameter and length of ZnO nanorods changed with heating methods and growth time. Moreover, fluorescence detection by ZnO nanorods, synthesized with different heating methods, were evaluated by FITC-labeled goat anti-bovine IgG. And the results revealed that ZnO nanorods synthesized on chip by localized heating showed better detection performance of fluorescein-labeled protein and good linearity from 10 pg/mL to 1 μg/mL with the correlation coefficient of 0.99209. The as-synthesized ZnO nanorods were further used to demonstrate detection of human α-fetoprotein (AFP), and achieved a superior limit of detection as low as 1 pg/mL. This work demonstrated that ZnO nanorods synthesized by microfluidic device allowed multiplexed fluorescence biodetection.