ZnS quantum dots derived a reagentless uric acid biosensor

Fenfen Zhang; Chenxin Li; Xiaohua Li; Xiaoli Wang; Qiao Wan; Yuezhong Xian; Litong Jin; Katsunobu Yamamoto

doi:10.1016/j.talanta.2005.07.051

ZnS quantum dots derived a reagentless uric acid biosensor

Fenfen Zhang, Chenxin Li, Xiaohua Li, Xiaoli Wang, Qiao Wan, Yuezhong Xian, Litong Jin, Katsunobu Yamamoto

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

A reagentless amperometric uric acid biosensor based on zinc sulfide (ZnS) quantum dots (QDs) was firstly developed. It could detect uric acid without the presence of an electron mediator. The carboxyl group functionalized ZnS QDs were synthesized, and they were soluble biocompatible and conductive. ZnS QDs conjugates could provide increased enzyme binding sites, which may result in higher enzyme loading. Thus, the proposed uricase/ZnS QDs/l-cys biosensor exhibited higher amperometric response compared to the one without QDs (uricase/l-cys biosensor). In addition, there was little AA interference. It showed a linear dependence on the uric acid concentration ranging from 5.0 × 10^-6 to 2.0 × 10^-3 mol L^-1 with a detection limit of 2.0 × 10^-6 mol L^-1 at 3σ.

Original language	English
Pages (from-to)	1353-1358
Number of pages	6
Journal	Talanta
Volume	68
Issue number	4
DOIs	https://doi.org/10.1016/j.talanta.2005.07.051
State	Published - 15 Feb 2006

Keywords

Reagentless biosensor
Uric acid
Uricase
ZnS quantum dots

Access to Document

10.1016/j.talanta.2005.07.051

Cite this

@article{cd263bc11d674c37a8f02a0452c3735a,

title = "ZnS quantum dots derived a reagentless uric acid biosensor",

abstract = "A reagentless amperometric uric acid biosensor based on zinc sulfide (ZnS) quantum dots (QDs) was firstly developed. It could detect uric acid without the presence of an electron mediator. The carboxyl group functionalized ZnS QDs were synthesized, and they were soluble biocompatible and conductive. ZnS QDs conjugates could provide increased enzyme binding sites, which may result in higher enzyme loading. Thus, the proposed uricase/ZnS QDs/l-cys biosensor exhibited higher amperometric response compared to the one without QDs (uricase/l-cys biosensor). In addition, there was little AA interference. It showed a linear dependence on the uric acid concentration ranging from 5.0 × 10-6 to 2.0 × 10-3 mol L-1 with a detection limit of 2.0 × 10-6 mol L-1 at 3σ.",

keywords = "Reagentless biosensor, Uric acid, Uricase, ZnS quantum dots",

author = "Fenfen Zhang and Chenxin Li and Xiaohua Li and Xiaoli Wang and Qiao Wan and Yuezhong Xian and Litong Jin and Katsunobu Yamamoto",

year = "2006",

month = feb,

day = "15",

doi = "10.1016/j.talanta.2005.07.051",

language = "英语",

volume = "68",

pages = "1353--1358",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier B.V.",

number = "4",

}

TY - JOUR

T1 - ZnS quantum dots derived a reagentless uric acid biosensor

AU - Zhang, Fenfen

AU - Li, Chenxin

AU - Li, Xiaohua

AU - Wang, Xiaoli

AU - Wan, Qiao

AU - Xian, Yuezhong

AU - Jin, Litong

AU - Yamamoto, Katsunobu

PY - 2006/2/15

Y1 - 2006/2/15

N2 - A reagentless amperometric uric acid biosensor based on zinc sulfide (ZnS) quantum dots (QDs) was firstly developed. It could detect uric acid without the presence of an electron mediator. The carboxyl group functionalized ZnS QDs were synthesized, and they were soluble biocompatible and conductive. ZnS QDs conjugates could provide increased enzyme binding sites, which may result in higher enzyme loading. Thus, the proposed uricase/ZnS QDs/l-cys biosensor exhibited higher amperometric response compared to the one without QDs (uricase/l-cys biosensor). In addition, there was little AA interference. It showed a linear dependence on the uric acid concentration ranging from 5.0 × 10-6 to 2.0 × 10-3 mol L-1 with a detection limit of 2.0 × 10-6 mol L-1 at 3σ.

AB - A reagentless amperometric uric acid biosensor based on zinc sulfide (ZnS) quantum dots (QDs) was firstly developed. It could detect uric acid without the presence of an electron mediator. The carboxyl group functionalized ZnS QDs were synthesized, and they were soluble biocompatible and conductive. ZnS QDs conjugates could provide increased enzyme binding sites, which may result in higher enzyme loading. Thus, the proposed uricase/ZnS QDs/l-cys biosensor exhibited higher amperometric response compared to the one without QDs (uricase/l-cys biosensor). In addition, there was little AA interference. It showed a linear dependence on the uric acid concentration ranging from 5.0 × 10-6 to 2.0 × 10-3 mol L-1 with a detection limit of 2.0 × 10-6 mol L-1 at 3σ.

KW - Reagentless biosensor

KW - Uric acid

KW - Uricase

KW - ZnS quantum dots

UR - https://www.scopus.com/pages/publications/30744463535

U2 - 10.1016/j.talanta.2005.07.051

DO - 10.1016/j.talanta.2005.07.051

M3 - 文章

AN - SCOPUS:30744463535

SN - 0039-9140

VL - 68

SP - 1353

EP - 1358

JO - Talanta

JF - Talanta

IS - 4

ER -

ZnS quantum dots derived a reagentless uric acid biosensor

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this