TY - JOUR
T1 - ZNF569, a novel KRAB-containing zinc finger protein, suppresses MAPK signaling pathway
AU - Huang, Xinqiong
AU - Yuan, Wuzhou
AU - Huang, Wen
AU - Bai, Yan
AU - Deng, Yun
AU - Zhu, Chuanbing
AU - Liang, Pei
AU - Li, Yongqing
AU - Du, Xiaoyan
AU - Liu, Mingyao
AU - Wang, Yuequn
AU - Wu, Xiushan
PY - 2006/8/4
Y1 - 2006/8/4
N2 - Transcription factors play an essential role in altering gene expression. A great progress about transcription factors has been made towards the understanding of normal physiological processes, embryonic development, and human diseases. Here we report the identification and characterization of a novel KRAB-containing zinc-finger protein, ZNF569, which is isolated from a human embryonic heart cDNA library. ZNF569 encodes a putative protein of 686 amino acids. The protein is conserved across different species during evolution. Expression of ZNF569 was found in most of the examined human adult and embryonic tissues with a higher level in heart and skeletal muscles. The KRAB and ZNF motifs of ZNF569 represent potent repression domains. When ZNF569 is fused to Gal-4 DNA-binding domain and co-transfected with VP-16, ZNF569 protein suppresses transcriptional activity. Overexpression of ZNF569 in COS-7 cells inhibited the transcriptional activities of SRE and AP-1, which may be silenced by siRNA. The results suggest that ZNF569 protein may act as a transcriptional repressor that suppresses MAPK signaling pathway to mediate cellular functions.
AB - Transcription factors play an essential role in altering gene expression. A great progress about transcription factors has been made towards the understanding of normal physiological processes, embryonic development, and human diseases. Here we report the identification and characterization of a novel KRAB-containing zinc-finger protein, ZNF569, which is isolated from a human embryonic heart cDNA library. ZNF569 encodes a putative protein of 686 amino acids. The protein is conserved across different species during evolution. Expression of ZNF569 was found in most of the examined human adult and embryonic tissues with a higher level in heart and skeletal muscles. The KRAB and ZNF motifs of ZNF569 represent potent repression domains. When ZNF569 is fused to Gal-4 DNA-binding domain and co-transfected with VP-16, ZNF569 protein suppresses transcriptional activity. Overexpression of ZNF569 in COS-7 cells inhibited the transcriptional activities of SRE and AP-1, which may be silenced by siRNA. The results suggest that ZNF569 protein may act as a transcriptional repressor that suppresses MAPK signaling pathway to mediate cellular functions.
KW - MAPK signaling pathway
KW - RNAi
KW - Transcriptional suppressor
KW - ZNF569
UR - https://www.scopus.com/pages/publications/33745191407
U2 - 10.1016/j.bbrc.2006.05.109
DO - 10.1016/j.bbrc.2006.05.109
M3 - 文章
C2 - 16793018
AN - SCOPUS:33745191407
SN - 0006-291X
VL - 346
SP - 621
EP - 628
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -
