Zebrafish genomic library in yeast artificial chromosomes

Tao P. Zhong; Kim Kaphingst; Uma Akella; Maryann Haldi; Eric S. Lander; Mark C. Fishman

doi:10.1006/geno.1997.5155

Zebrafish genomic library in yeast artificial chromosomes

Tao P. Zhong
, Kim Kaphingst
, Uma Akella
, Maryann Haldi
, Eric S. Lander
, Mark C. Fishman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We have constructed a zebrafish yeast artificial chromosome (YAC) library using genomic DNA isolated from the inbred AB zebrafish strain. The average insert size is 470 kb, estimated from analysis of 155 random selected YACs. The library consists of 17,000 clones, providing about a 4.7-fold coverage of zebrafish genome. The YAC clones have been arrayed in individual wells of 96-well microplates and also pooled to permit rapid polymerase chain reaction screening of the entire library. We have also found that the YAC ends can be easily rescued and sequenced from pRML1/pRML2-based mini-YAC clones.

Original language	English
Pages (from-to)	136-138
Number of pages	3
Journal	Genomics
Volume	48
Issue number	1
DOIs	https://doi.org/10.1006/geno.1997.5155
State	Published - 15 Feb 1998
Externally published	Yes

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10.1006/geno.1997.5155

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title = "Zebrafish genomic library in yeast artificial chromosomes",

abstract = "We have constructed a zebrafish yeast artificial chromosome (YAC) library using genomic DNA isolated from the inbred AB zebrafish strain. The average insert size is 470 kb, estimated from analysis of 155 random selected YACs. The library consists of 17,000 clones, providing about a 4.7-fold coverage of zebrafish genome. The YAC clones have been arrayed in individual wells of 96-well microplates and also pooled to permit rapid polymerase chain reaction screening of the entire library. We have also found that the YAC ends can be easily rescued and sequenced from pRML1/pRML2-based mini-YAC clones.",

author = "Zhong, \{Tao P.\} and Kim Kaphingst and Uma Akella and Maryann Haldi and Lander, \{Eric S.\} and Fishman, \{Mark C.\}",

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AU - Zhong, Tao P.

AU - Kaphingst, Kim

AU - Akella, Uma

AU - Haldi, Maryann

AU - Lander, Eric S.

AU - Fishman, Mark C.

PY - 1998/2/15

Y1 - 1998/2/15

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AB - We have constructed a zebrafish yeast artificial chromosome (YAC) library using genomic DNA isolated from the inbred AB zebrafish strain. The average insert size is 470 kb, estimated from analysis of 155 random selected YACs. The library consists of 17,000 clones, providing about a 4.7-fold coverage of zebrafish genome. The YAC clones have been arrayed in individual wells of 96-well microplates and also pooled to permit rapid polymerase chain reaction screening of the entire library. We have also found that the YAC ends can be easily rescued and sequenced from pRML1/pRML2-based mini-YAC clones.

UR - https://www.scopus.com/pages/publications/0031913262

U2 - 10.1006/geno.1997.5155

DO - 10.1006/geno.1997.5155

M3 - 文章

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SP - 136

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JO - Genomics

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Zebrafish genomic library in yeast artificial chromosomes

Abstract

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