Visualizing dopamine released from living cells using a nanoplasmonic probe

W. W. Qin; S. P. Wang; J. Li; T. H. Peng; Y. Xu; K. Wang; J. Y. Shi; C. H. Fan; D. Li

doi:10.1039/c5nr04433b

Visualizing dopamine released from living cells using a nanoplasmonic probe

W. W. Qin, S. P. Wang, J. Li, T. H. Peng, Y. Xu, K. Wang, J. Y. Shi, C. H. Fan, D. Li^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We report the development of an ultrasensitive nanoplasmonic probe for discriminative detection and imaging of dopamine released from living cells. The sensing mechanism is based on the dopamine-induced seeded-growth of Au nanoparticles (Au NPs) that leads to the shift of the plasmon band. This platform allows for the detection of dopamine with a detection limit down to 0.25 pM within 1 min. This nanoplasmonic assay is further applied to visualize the release of dopamine from living rat pheochromocytoma (PC12) cells under ATP-stimulation with dark-field microscopy (DFM). The DFM results together with real time fluorescence imaging of PC12 cells stained with the Fluo calcium indicator, suggested that ATP stimulated-release of dopamine is concomitant with the Ca²⁺ influx, and the influx of Ca²⁺ is through ATP-activated channels instead of the voltage-gated Ca²⁺ channel (VGC).

Original language	English
Pages (from-to)	15070-15074
Number of pages	5
Journal	Nanoscale
Volume	7
Issue number	37
DOIs	https://doi.org/10.1039/c5nr04433b
State	Published - 7 Oct 2015
Externally published	Yes

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title = "Visualizing dopamine released from living cells using a nanoplasmonic probe",

abstract = "We report the development of an ultrasensitive nanoplasmonic probe for discriminative detection and imaging of dopamine released from living cells. The sensing mechanism is based on the dopamine-induced seeded-growth of Au nanoparticles (Au NPs) that leads to the shift of the plasmon band. This platform allows for the detection of dopamine with a detection limit down to 0.25 pM within 1 min. This nanoplasmonic assay is further applied to visualize the release of dopamine from living rat pheochromocytoma (PC12) cells under ATP-stimulation with dark-field microscopy (DFM). The DFM results together with real time fluorescence imaging of PC12 cells stained with the Fluo calcium indicator, suggested that ATP stimulated-release of dopamine is concomitant with the Ca2+ influx, and the influx of Ca2+ is through ATP-activated channels instead of the voltage-gated Ca2+ channel (VGC).",

author = "Qin, \{W. W.\} and Wang, \{S. P.\} and J. Li and Peng, \{T. H.\} and Y. Xu and K. Wang and Shi, \{J. Y.\} and Fan, \{C. H.\} and D. Li",

note = "Publisher Copyright: {\textcopyright} 2015 The Royal Society of Chemistry.",

year = "2015",

month = oct,

day = "7",

doi = "10.1039/c5nr04433b",

language = "英语",

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T1 - Visualizing dopamine released from living cells using a nanoplasmonic probe

AU - Qin, W. W.

AU - Wang, S. P.

AU - Li, J.

AU - Peng, T. H.

AU - Xu, Y.

AU - Wang, K.

AU - Shi, J. Y.

AU - Fan, C. H.

AU - Li, D.

PY - 2015/10/7

Y1 - 2015/10/7

N2 - We report the development of an ultrasensitive nanoplasmonic probe for discriminative detection and imaging of dopamine released from living cells. The sensing mechanism is based on the dopamine-induced seeded-growth of Au nanoparticles (Au NPs) that leads to the shift of the plasmon band. This platform allows for the detection of dopamine with a detection limit down to 0.25 pM within 1 min. This nanoplasmonic assay is further applied to visualize the release of dopamine from living rat pheochromocytoma (PC12) cells under ATP-stimulation with dark-field microscopy (DFM). The DFM results together with real time fluorescence imaging of PC12 cells stained with the Fluo calcium indicator, suggested that ATP stimulated-release of dopamine is concomitant with the Ca2+ influx, and the influx of Ca2+ is through ATP-activated channels instead of the voltage-gated Ca2+ channel (VGC).

AB - We report the development of an ultrasensitive nanoplasmonic probe for discriminative detection and imaging of dopamine released from living cells. The sensing mechanism is based on the dopamine-induced seeded-growth of Au nanoparticles (Au NPs) that leads to the shift of the plasmon band. This platform allows for the detection of dopamine with a detection limit down to 0.25 pM within 1 min. This nanoplasmonic assay is further applied to visualize the release of dopamine from living rat pheochromocytoma (PC12) cells under ATP-stimulation with dark-field microscopy (DFM). The DFM results together with real time fluorescence imaging of PC12 cells stained with the Fluo calcium indicator, suggested that ATP stimulated-release of dopamine is concomitant with the Ca2+ influx, and the influx of Ca2+ is through ATP-activated channels instead of the voltage-gated Ca2+ channel (VGC).

UR - https://www.scopus.com/pages/publications/84942154728

U2 - 10.1039/c5nr04433b

DO - 10.1039/c5nr04433b

M3 - 文章

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VL - 7

SP - 15070

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JO - Nanoscale

JF - Nanoscale

IS - 37

ER -

Visualizing dopamine released from living cells using a nanoplasmonic probe

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